Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P3T49_RS02320 Genome accession   NZ_CP120723
Coordinates   432099..432536 (-) Length   145 a.a.
NCBI ID   WP_088116478.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain NX-12     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 427099..437536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3T49_RS02285 - 427665..428120 (+) 456 WP_088116474.1 RDD family protein -
  P3T49_RS02290 - 428205..428534 (+) 330 WP_277714314.1 YckD family protein -
  P3T49_RS02295 - 428638..429063 (+) 426 WP_003328070.1 MarR family winged helix-turn-helix transcriptional regulator -
  P3T49_RS02300 - 429051..429392 (+) 342 WP_094231349.1 DUF3147 family protein -
  P3T49_RS02305 - 429412..430140 (+) 729 WP_106360828.1 AAA family ATPase -
  P3T49_RS02310 - 430210..431646 (+) 1437 WP_094231350.1 glycoside hydrolase family 1 protein -
  P3T49_RS02315 nin/comJ 431682..432080 (-) 399 WP_003328075.1 competence protein ComJ Regulator
  P3T49_RS02320 nucA/comI 432099..432536 (-) 438 WP_088116478.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  P3T49_RS02325 - 432758..434461 (-) 1704 WP_277714315.1 methyl-accepting chemotaxis protein -
  P3T49_RS02330 hxlB 434590..435147 (-) 558 WP_010787607.1 6-phospho-3-hexuloisomerase -
  P3T49_RS02335 hxlA 435154..435786 (-) 633 WP_088116480.1 3-hexulose-6-phosphate synthase -
  P3T49_RS02340 - 436004..436369 (+) 366 WP_003328081.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16005.77 Da        Isoelectric Point: 4.1626

>NTDB_id=808332 P3T49_RS02320 WP_088116478.1 432099..432536(-) (nucA/comI) [Bacillus atrophaeus strain NX-12]
MDLLKSLLLILVIIAAAIAGLVKGDFFSENQKASEDKEYDETISFPSDRYPETAKHIQDAINEGESDVCTIDRDGAEERR
EQSLKDVAVKTGYDRDEWPMAMCKEGGEGASVEYIAPSDNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 438 bp        

>NTDB_id=808332 P3T49_RS02320 WP_088116478.1 432099..432536(-) (nucA/comI) [Bacillus atrophaeus strain NX-12]
ATGGACTTATTGAAATCATTGCTGCTCATATTGGTGATCATTGCTGCCGCCATTGCCGGGCTGGTTAAAGGCGATTTTTT
CTCAGAAAATCAAAAGGCGTCTGAAGACAAAGAGTATGACGAAACGATTTCTTTCCCATCAGATCGTTATCCTGAAACTG
CGAAACATATTCAAGATGCGATCAATGAAGGTGAGTCAGATGTGTGCACAATTGACAGGGACGGGGCTGAAGAACGACGA
GAACAATCTTTAAAAGATGTGGCAGTCAAAACAGGATACGATCGGGACGAATGGCCGATGGCAATGTGTAAAGAAGGCGG
AGAAGGTGCGTCTGTGGAGTATATCGCCCCGTCGGACAATCGCGGGGCTGGCTCATGGGTCGGTCATCAGCTCACTGATT
ATCCAGATGGAACGAAAGTGTTATTTACCATTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

82.639

99.31

0.821