Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   TSUB_RS01060 Genome accession   NZ_AP023054
Coordinates   221014..222654 (+) Length   546 a.a.
NCBI ID   WP_087020818.1    Uniprot ID   -
Organism   Thaumasiovibrio subtropicus strain C4V358     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 216014..227654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TSUB_RS01040 pilM 218158..219153 (+) 996 WP_087020784.1 type IV pilus assembly protein PilM -
  TSUB_RS01045 - 219150..219710 (+) 561 WP_087020787.1 PilN domain-containing protein -
  TSUB_RS01050 - 219710..220291 (+) 582 WP_087020790.1 type 4a pilus biogenesis protein PilO -
  TSUB_RS01055 - 220281..220817 (+) 537 WP_087020793.1 pilus assembly protein PilP -
  TSUB_RS01060 pilQ 221014..222654 (+) 1641 WP_087020818.1 type IV pilus secretin PilQ Machinery gene
  TSUB_RS01065 aroK 222814..223332 (+) 519 WP_087020796.1 shikimate kinase AroK -
  TSUB_RS01070 aroB 223355..224431 (+) 1077 WP_087020799.1 3-dehydroquinate synthase -
  TSUB_RS01075 - 224443..226092 (+) 1650 WP_087020802.1 SPOR domain-containing protein -
  TSUB_RS01080 - 226164..227000 (+) 837 WP_087020805.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  TSUB_RS01085 - 227011..227172 (-) 162 WP_087020821.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 546 a.a.        Molecular weight: 59541.61 Da        Isoelectric Point: 4.2305

>NTDB_id=80827 TSUB_RS01060 WP_087020818.1 221014..222654(+) (pilQ) [Thaumasiovibrio subtropicus strain C4V358]
MPNTNSVENIDFRRDKDGNGVLIAEFANPQAIVNVERRKQALQVDVLNTALPNELLYILDVNDFATVVSDVETFLLEDGA
RLVLSTKGDYEYDYQLNGKQLRVVMKQPPRQEVATAQSQVETVKYTGEPISINFQDIPVRNVLQLVADYNDFNLVVSDSV
VGNVTLRLDGVPWEQVLDLILRVRGLDKRVEGNVVLVAPKAELEKNELEALEAQKMEEELAPLETELYSINFAKAGDIAA
LLAGTEDGISLLTDRGAVSTDERTNAVIVRDVPAQQEVIAKLISDLDIAIKQVQIEARIVTVDEGDLSALGVKWGFSNTN
GSVAGGGSIESNIWAMPGRSIEGKDSVEMDDYLNVNLGIADPRASSIAFSVAKLGKNLLLDLELSALQAENKAEIVSSPR
LMTADKKPAYIEQGVEIPYLESASSGAATIAFQKAVLSLRVVPQITPDGRLILDLEVTQDSQGDTVDTGFGKAVSINTRR
IDTQVMVEDGETVVLGGIFQHAITKTTDKVPVLGDLPALGMLFRRDSENIQKREMLIFVTPQIVSQ

Nucleotide


Download         Length: 1641 bp        

>NTDB_id=80827 TSUB_RS01060 WP_087020818.1 221014..222654(+) (pilQ) [Thaumasiovibrio subtropicus strain C4V358]
ATGCCAAACACTAATAGTGTTGAGAACATTGACTTTCGACGAGATAAAGATGGTAACGGTGTACTGATTGCTGAGTTTGC
GAATCCACAAGCGATCGTCAATGTAGAACGTCGAAAGCAAGCACTACAGGTCGATGTTCTTAATACCGCTCTCCCCAACG
AGCTGCTTTATATTTTAGATGTTAACGACTTTGCGACCGTGGTAAGTGATGTCGAAACCTTCTTATTAGAAGATGGTGCG
CGTCTGGTGTTGAGCACAAAAGGCGACTATGAGTATGACTACCAGCTTAATGGCAAGCAGTTGCGCGTCGTCATGAAGCA
GCCGCCTCGCCAAGAAGTTGCGACAGCACAGAGCCAAGTTGAAACGGTGAAGTACACTGGCGAACCCATTTCTATCAACT
TTCAAGATATACCAGTGCGAAATGTGCTTCAGTTAGTCGCTGATTACAATGATTTTAACTTGGTGGTCTCTGATAGTGTT
GTTGGTAATGTCACCCTCCGTCTAGATGGCGTGCCTTGGGAACAAGTGCTGGATCTTATCTTACGCGTAAGGGGTCTAGA
TAAACGTGTTGAAGGTAACGTTGTTTTGGTTGCGCCAAAAGCCGAGCTTGAAAAGAACGAGCTCGAAGCCTTGGAAGCAC
AGAAGATGGAAGAGGAGTTAGCGCCACTAGAGACCGAGCTCTATTCGATTAACTTTGCTAAGGCAGGAGACATTGCAGCG
TTGTTGGCTGGTACAGAAGATGGTATTAGCTTACTGACTGACCGTGGTGCAGTGAGTACCGATGAACGCACTAATGCAGT
GATTGTTCGAGATGTGCCTGCGCAACAGGAAGTGATTGCAAAGCTGATCAGTGACCTTGATATTGCGATTAAGCAAGTGC
AAATTGAGGCTCGCATTGTGACGGTGGATGAGGGTGACCTCTCTGCGTTGGGTGTGAAATGGGGCTTCTCAAATACTAAT
GGCAGTGTGGCTGGAGGCGGTAGTATTGAGAGTAACATTTGGGCGATGCCAGGACGTTCGATAGAAGGTAAAGATTCGGT
CGAAATGGATGACTATCTCAATGTGAACCTCGGTATTGCTGACCCACGAGCGTCCAGTATTGCATTCAGTGTTGCCAAAT
TGGGTAAAAACTTACTGTTGGACCTCGAACTTTCTGCGCTGCAAGCGGAAAACAAGGCGGAGATTGTCTCTAGTCCACGT
CTTATGACGGCAGACAAGAAACCGGCTTACATAGAGCAGGGTGTCGAAATCCCGTATTTGGAATCTGCATCGAGCGGCGC
AGCCACCATTGCGTTCCAAAAAGCGGTGTTGAGTCTTCGCGTCGTACCACAAATCACGCCGGATGGACGTTTGATTCTTG
ACCTTGAGGTGACTCAAGATAGTCAGGGTGACACGGTTGATACTGGTTTTGGTAAAGCTGTATCTATCAACACGCGTCGA
ATTGACACCCAAGTTATGGTCGAAGATGGTGAAACCGTGGTCTTAGGCGGAATTTTCCAGCATGCCATCACGAAAACGAC
GGACAAGGTGCCTGTGCTAGGGGATTTACCTGCACTGGGTATGCTATTCCGCCGAGATTCTGAGAATATTCAAAAGCGTG
AGATGCTGATCTTTGTAACGCCGCAGATTGTTAGCCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(386-544)

Bacterial type II/III secretion system short domain (Secretin_N)

(226-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

59.041

99.267

0.586
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

59.041

99.267

0.586
  pilQ Vibrio campbellii strain DS40M4

58.394

100

0.586
  comQ Acinetobacter baylyi ADP1

40.246

100

0.419
  pilQ Acinetobacter baumannii strain A118

38.832

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.832

100

0.414
  pilQ Legionella pneumophila strain Lp02

39.599

100

0.397
  pilQ Pseudomonas aeruginosa PAK

38.959

100

0.397
  pilQ Legionella pneumophila strain ERS1305867

39.416

100

0.396

Multiple sequence alignment