Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   P0M29_RS04540 Genome accession   NZ_CP120711
Coordinates   938925..939539 (-) Length   204 a.a.
NCBI ID   WP_029973402.1    Uniprot ID   -
Organism   Bacillus velezensis strain 12Y     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 933925..944539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P0M29_RS04510 (P0M29_04510) rpsT 934228..934494 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  P0M29_RS04515 (P0M29_04515) holA 934457..935552 (-) 1096 Protein_899 DNA polymerase III subunit delta -
  P0M29_RS04520 (P0M29_04520) - 935592..935743 (-) 152 Protein_900 hypothetical protein -
  P0M29_RS04525 (P0M29_04525) - 935784..935918 (+) 135 WP_003152870.1 YqzM family protein -
  P0M29_RS04530 (P0M29_04530) comEC 935937..938288 (-) 2352 WP_029973403.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  P0M29_RS04535 (P0M29_04535) - 938289..938858 (-) 570 WP_003152868.1 ComE operon protein 2 -
  P0M29_RS04540 (P0M29_04540) comEA 938925..939539 (-) 615 WP_029973402.1 helix-hairpin-helix domain-containing protein Machinery gene
  P0M29_RS04545 (P0M29_04545) comER 939598..940419 (+) 822 WP_029973401.1 late competence protein ComER -
  P0M29_RS04550 (P0M29_04550) - 940488..941225 (-) 738 WP_015417856.1 class I SAM-dependent methyltransferase -
  P0M29_RS04555 (P0M29_04555) rsfS 941222..941578 (-) 357 WP_007408260.1 ribosome silencing factor -
  P0M29_RS04560 (P0M29_04560) yqeK 941596..942156 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  P0M29_RS04565 (P0M29_04565) - 942146..942715 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  P0M29_RS04570 (P0M29_04570) yhbY 942726..943016 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  P0M29_RS04575 (P0M29_04575) aroE 943010..943852 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22240.29 Da        Isoelectric Point: 5.0355

>NTDB_id=808161 P0M29_RS04540 WP_029973402.1 938925..939539(-) (comEA) [Bacillus velezensis strain 12Y]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=808161 P0M29_RS04540 WP_029973402.1 938925..939539(-) (comEA) [Bacillus velezensis strain 12Y]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363