Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   P3X63_RS14085 Genome accession   NZ_CP120679
Coordinates   2721017..2721646 (-) Length   209 a.a.
NCBI ID   WP_277691098.1    Uniprot ID   -
Organism   Bacillus sp. HSf4     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2716017..2726646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3X63_RS14065 (P3X63_14065) holA 2716542..2717564 (-) 1023 WP_026587933.1 DNA polymerase III subunit delta -
  P3X63_RS14070 (P3X63_14070) - 2717799..2717930 (+) 132 WP_026587934.1 YqzM family protein -
  P3X63_RS14075 (P3X63_14075) comEC 2718042..2720372 (-) 2331 WP_277691095.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  P3X63_RS14080 (P3X63_14080) - 2720379..2720948 (-) 570 WP_077736398.1 ComE operon protein 2 -
  P3X63_RS14085 (P3X63_14085) comEA 2721017..2721646 (-) 630 WP_277691098.1 helix-hairpin-helix domain-containing protein Machinery gene
  P3X63_RS14090 (P3X63_14090) comER 2721709..2722551 (+) 843 WP_256860380.1 late competence protein ComER -
  P3X63_RS14095 (P3X63_14095) - 2722619..2723374 (-) 756 WP_277691101.1 class I SAM-dependent methyltransferase -
  P3X63_RS14100 (P3X63_14100) rsfS 2723374..2723727 (-) 354 WP_026587940.1 ribosome silencing factor -
  P3X63_RS14105 (P3X63_14105) yqeK 2723741..2724304 (-) 564 WP_026587941.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  P3X63_RS14110 (P3X63_14110) - 2724294..2724863 (-) 570 WP_026587942.1 nicotinate-nucleotide adenylyltransferase -
  P3X63_RS14115 (P3X63_14115) yhbY 2724884..2725174 (-) 291 WP_026587943.1 ribosome assembly RNA-binding protein YhbY -
  P3X63_RS14120 (P3X63_14120) aroE 2725168..2726004 (-) 837 WP_026587944.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22451.33 Da        Isoelectric Point: 4.7922

>NTDB_id=807675 P3X63_RS14085 WP_277691098.1 2721017..2721646(-) (comEA) [Bacillus sp. HSf4]
MLNWLKQYKWFAAGGLALVVIIAAAVVTKGGDDLSSAHSSVPDEALQSPEKTASLPEDKKNEEKEEIVIDLKGAVKQPGV
YEMKAGDRVHHLLKKAGGTVKKADQKQVNLAAILRDGMVVYIPFQGEEASAHSESGSYLAEGEEENTININTASAKELED
IPGVGPSKAEAIVTYREENGPFQTIEDITNVSGIGEKSFEKIKSSISVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=807675 P3X63_RS14085 WP_277691098.1 2721017..2721646(-) (comEA) [Bacillus sp. HSf4]
ATGCTAAATTGGCTCAAACAATACAAATGGTTTGCGGCAGGGGGATTGGCGCTAGTGGTCATCATTGCCGCCGCAGTCGT
CACAAAGGGCGGTGACGATCTATCCTCAGCTCATTCGAGTGTGCCGGATGAAGCTTTGCAATCACCTGAGAAGACGGCAA
GTTTGCCAGAGGATAAGAAAAACGAAGAAAAAGAAGAAATCGTCATCGATCTGAAGGGGGCGGTCAAGCAGCCCGGTGTT
TACGAAATGAAGGCAGGTGACAGAGTTCATCATTTACTAAAAAAAGCGGGCGGCACCGTGAAAAAAGCCGATCAAAAACA
AGTCAACCTAGCCGCCATTTTACGGGACGGCATGGTCGTTTATATTCCGTTTCAAGGAGAAGAAGCCTCCGCTCATTCTG
AATCAGGGTCGTATTTGGCAGAAGGAGAAGAGGAAAACACGATCAATATCAACACAGCTTCTGCAAAAGAGCTTGAGGAC
ATTCCCGGTGTTGGCCCTTCAAAAGCGGAAGCGATCGTCACATATCGGGAGGAAAACGGCCCATTTCAGACGATAGAAGA
CATCACCAATGTGTCAGGTATTGGAGAAAAATCATTTGAAAAAATAAAATCGTCAATATCGGTAAAGTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(69-123)

Helix-hairpin-helix motif (HHH_3)

(145-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

53.589

100

0.536
  comEA Staphylococcus aureus N315

36.239

100

0.378
  comEA Staphylococcus aureus MW2

36.073

100

0.378