Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   P5628_RS12825 Genome accession   NZ_CP120577
Coordinates   2467334..2467951 (-) Length   205 a.a.
NCBI ID   WP_277708803.1    Uniprot ID   -
Organism   Bacillus subtilis strain PRO53     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2462334..2472951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5628_RS12795 (P5628_12795) rpsT 2462635..2462901 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  P5628_RS12800 (P5628_12800) holA 2462916..2463959 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  P5628_RS12805 (P5628_12805) - 2463999..2464112 (-) 114 WP_122060479.1 hypothetical protein -
  P5628_RS12810 (P5628_12810) yqzM 2464189..2464323 (+) 135 WP_003229983.1 YqzM family protein -
  P5628_RS12815 (P5628_12815) comEC 2464364..2466694 (-) 2331 WP_249848131.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  P5628_RS12820 (P5628_12820) comEB 2466698..2467267 (-) 570 WP_072174291.1 ComE operon protein 2 -
  P5628_RS12825 (P5628_12825) comEA 2467334..2467951 (-) 618 WP_277708803.1 competence protein ComEA Machinery gene
  P5628_RS12830 (P5628_12830) comER 2468035..2468856 (+) 822 WP_021480064.1 late competence protein ComER -
  P5628_RS12835 (P5628_12835) yqeM 2468922..2469665 (-) 744 WP_070547553.1 class I SAM-dependent methyltransferase -
  P5628_RS12840 (P5628_12840) rsfS 2469662..2470018 (-) 357 WP_014480315.1 ribosome silencing factor -
  P5628_RS12845 (P5628_12845) yqeK 2470036..2470596 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  P5628_RS12850 (P5628_12850) nadD 2470586..2471155 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  P5628_RS12855 (P5628_12855) yhbY 2471167..2471457 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  P5628_RS12860 (P5628_12860) aroE 2471451..2472293 (-) 843 WP_070547554.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21838.60 Da        Isoelectric Point: 4.8417

>NTDB_id=806317 P5628_RS12825 WP_277708803.1 2467334..2467951(-) (comEA) [Bacillus subtilis strain PRO53]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVIKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=806317 P5628_RS12825 WP_277708803.1 2467334..2467951(-) (comEA) [Bacillus subtilis strain PRO53]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGATAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGAATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38