Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   P3G65_RS05115 Genome accession   NZ_CP120417
Coordinates   949714..950970 (-) Length   418 a.a.
NCBI ID   WP_019292770.1    Uniprot ID   -
Organism   Lactococcus sp. bn62     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 944714..955970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3G65_RS05085 (P3G65_05095) ald 945467..946573 (+) 1107 WP_019292776.1 alanine dehydrogenase -
  P3G65_RS05090 (P3G65_05100) - 946693..947652 (+) 960 WP_019292775.1 PhoH family protein -
  P3G65_RS05095 (P3G65_05105) - 947654..948127 (+) 474 WP_019292774.1 NUDIX hydrolase -
  P3G65_RS05100 (P3G65_05110) ybeY 948137..948625 (+) 489 WP_019292773.1 rRNA maturation RNase YbeY -
  P3G65_RS05105 (P3G65_05115) - 948609..949070 (+) 462 WP_019292772.1 diacylglycerol kinase family protein -
  P3G65_RS05110 (P3G65_05120) - 949073..949717 (-) 645 WP_019292771.1 ComF family protein -
  P3G65_RS05115 (P3G65_05125) comFA 949714..950970 (-) 1257 WP_019292770.1 DEAD/DEAH box helicase Machinery gene
  P3G65_RS05120 (P3G65_05130) - 951025..951648 (+) 624 WP_019292769.1 YigZ family protein -
  P3G65_RS05125 (P3G65_05135) - 951661..952683 (+) 1023 WP_019292768.1 DUF475 domain-containing protein -
  P3G65_RS05130 (P3G65_05140) - 952718..953740 (-) 1023 WP_034999659.1 alpha/beta hydrolase -
  P3G65_RS05135 (P3G65_05145) - 954058..954837 (+) 780 WP_019292766.1 formate/nitrite transporter family protein -
  P3G65_RS05140 (P3G65_05150) glyQ 954851..955819 (+) 969 WP_019292765.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 48043.96 Da        Isoelectric Point: 10.1835

>NTDB_id=805562 P3G65_RS05115 WP_019292770.1 949714..950970(-) (comFA) [Lactococcus sp. bn62]
MNELYGRLLLQKELSKIPDKATLFDSMHDISRTVIMCNRCGKKSKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FLAASFLNWQGKLTAPQQYISDTLVKLHQQQKTVLVQAVTGAGKTEMIYQMIDNTLKKGKAVALTSPRIDVCLELYYRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLLIFDEVDAFPFPDNDMLSFALTQARKPSSSLIYLTATTTDN
LERQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRLGEKLNQDLQSSFPHEKIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPDGKVFFFHLGKITA
MLEAYKNIRNMNKAGGFQ

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=805562 P3G65_RS05115 WP_019292770.1 949714..950970(-) (comFA) [Lactococcus sp. bn62]
ATGAATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGTCGAAAATCCCAGACAAAGCAACACTTTTTGATAGTAT
GCACGATATTTCAAGAACAGTGATTATGTGCAATCGCTGTGGCAAAAAAAGTAAGAAAAAAGAGGTCCTACTCCCCGTTG
GTGCTTATTATTGTCCCCATTGTATTCAGATGGGACGTGTTCGCTCGGATGAAAAACTTTATCATCTTCCTCAGGAGGAT
TTTTTAGCAGCTTCTTTTCTAAACTGGCAGGGAAAACTCACAGCCCCTCAGCAATATATATCTGATACTCTAGTCAAATT
ACATCAGCAACAAAAAACGGTGCTGGTACAGGCTGTCACGGGTGCAGGAAAAACTGAAATGATTTATCAGATGATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGCTTTAACTAGCCCTCGTATTGATGTCTGTCTAGAACTCTACTATCGCTTAAAG
CGAGATTTTTCTTGTCCCATCTCGCTTTTACACGGAAAAAGTGAGAAATACAGCCGCTCGCCACTCGTAATTGCGACGAC
ACATCAGCTTATGCGTTTTCGTCATGCCTTTGACTTACTCATTTTTGATGAGGTTGATGCTTTTCCATTTCCAGATAATG
ACATGCTTTCTTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCGATAAT
TTGGAAAGACAAGTCAAGTTGGGGCAAATCGAAAAGTTACAACTTCCCCGACGTTTTCATGGTTTTCCATTGGTACTCCC
ACAATTTTTTTGGCAGAGCAAGTTTTATAAAATGGTCAAAAAACAACGCGAATCTGGTTTCCCATTACTTATCTTTGTCC
CTGAAATAAGACTGGGAGAAAAACTGAACCAAGACTTACAGAGTAGTTTTCCTCATGAAAAAATTGCCTTTGTTGCTTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGTTTTCGCCAGGGCAATATTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACTTTTCCTAAGGTTGACGTTTTTGTATTTCAAAGTCATCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCTGGACGAGTAGGTCGAAGTACCGAAAGACCTGACGGCAAAGTGTTTTTCTTTCATTTAGGAAAAATAACAGCA
ATGTTGGAAGCTTATAAAAATATCAGAAATATGAATAAGGCAGGAGGTTTCCAATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(97-236)

Helicase conserved C-terminal domain (Helicase_C)

(274-382)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

57.398

93.78

0.538
  comFA/cflA Streptococcus mitis SK321

51.288

100

0.524
  comFA/cflA Streptococcus pneumoniae TIGR4

50.82

100

0.519
  comFA/cflA Streptococcus pneumoniae Rx1

50.82

100

0.519
  comFA/cflA Streptococcus pneumoniae D39

50.82

100

0.519
  comFA/cflA Streptococcus pneumoniae R6

50.82

100

0.519
  comFA/cflA Streptococcus mitis NCTC 12261

50.351

100

0.514
  comFA Bacillus subtilis subsp. subtilis str. 168

37.443

100

0.392
  comFA Latilactobacillus sakei subsp. sakei 23K

38.791

94.976

0.368