Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   P2T68_RS05530 Genome accession   NZ_CP120377
Coordinates   1222996..1223862 (-) Length   288 a.a.
NCBI ID   WP_069075202.1    Uniprot ID   A0AB36Y547
Organism   Pseudomonas sp. G11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1217996..1228862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P2T68_RS05490 (P2T68_05490) - 1218385..1219683 (-) 1299 WP_168232462.1 NAD(P)/FAD-dependent oxidoreductase -
  P2T68_RS05495 (P2T68_05495) - 1219744..1219917 (-) 174 WP_024073379.1 DUF3094 family protein -
  P2T68_RS05500 (P2T68_05500) - 1220007..1220636 (-) 630 WP_017845475.1 DUF1780 domain-containing protein -
  P2T68_RS05505 (P2T68_05505) - 1220737..1221204 (+) 468 WP_277379131.1 FAD/FMN-containing dehydrogenase -
  P2T68_RS05510 (P2T68_05510) - 1221201..1221890 (+) 690 WP_024073381.1 energy-coupling factor ABC transporter permease -
  P2T68_RS05515 (P2T68_05515) - 1221967..1222182 (+) 216 WP_003171680.1 hypothetical protein -
  P2T68_RS05520 (P2T68_05520) yacG 1222179..1222379 (-) 201 WP_017845472.1 DNA gyrase inhibitor YacG -
  P2T68_RS05525 (P2T68_05525) coaE 1222376..1222999 (-) 624 WP_102624199.1 dephospho-CoA kinase -
  P2T68_RS05530 (P2T68_05530) pilD 1222996..1223862 (-) 867 WP_069075202.1 A24 family peptidase Machinery gene
  P2T68_RS05535 (P2T68_05535) pilC 1223859..1225067 (-) 1209 WP_193690171.1 type II secretion system F family protein Machinery gene
  P2T68_RS05540 (P2T68_05540) - 1225269..1225682 (+) 414 WP_024073385.1 pilin -
  P2T68_RS05545 (P2T68_05545) - 1225717..1227126 (+) 1410 WP_277379132.1 O-antigen ligase family protein -
  P2T68_RS05555 (P2T68_05555) - 1227389..1227694 (+) 306 WP_024073387.1 DUF6388 family protein -
  P2T68_RS35160 - 1227719..1227808 (+) 90 Protein_1088 outer membrane lipoprotein carrier protein LolA -
  P2T68_RS05560 (P2T68_05560) nadC 1227816..1228664 (-) 849 WP_150054127.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31860.16 Da        Isoelectric Point: 7.0716

>NTDB_id=805289 P2T68_RS05530 WP_069075202.1 1222996..1223862(-) (pilD) [Pseudomonas sp. G11]
MNLLLTEQPWAFVAVALVLGLIIGSFLNVLVWRLPKMLEREWRAQALEVLGMPADPAGPTYNLMHPNSCCPHCNHPIRPW
ENIPLLSYLMLRGRCAHCQQPISARYPITELACGLISAMVAWHFGFGWQAGAVMLLSWGLLAMSLIDVDHQLLPDILVLP
LLWLGLIINSADLLTALPDALWGAVIGYLSLWTVFWLFKLVTGKDGMGHGDFKLLALLGAWGGWQILPMTLLMSSLVGVI
VGLILMRLRKAEISAPMPFGPYLAIAGWIAVLWGGQITDFYLQSVGFR

Nucleotide


Download         Length: 867 bp        

>NTDB_id=805289 P2T68_RS05530 WP_069075202.1 1222996..1223862(-) (pilD) [Pseudomonas sp. G11]
TTGAACCTGCTGCTGACTGAGCAGCCATGGGCGTTTGTCGCAGTGGCGCTGGTACTGGGGCTGATCATCGGTAGCTTCCT
CAATGTACTGGTATGGCGCCTGCCGAAAATGCTTGAGCGGGAATGGCGCGCACAAGCCCTTGAAGTGCTCGGCATGCCCG
CCGACCCCGCCGGGCCCACCTACAACCTGATGCACCCCAACTCCTGCTGCCCGCACTGCAATCACCCGATTCGCCCGTGG
GAGAATATCCCGCTGCTCAGCTACCTCATGCTCCGGGGGCGTTGCGCCCATTGTCAGCAGCCGATCAGTGCACGCTACCC
CATCACTGAACTGGCCTGCGGGCTGATCTCGGCCATGGTGGCCTGGCATTTTGGCTTTGGCTGGCAAGCCGGGGCAGTCA
TGCTGTTGAGCTGGGGGCTGCTGGCAATGAGCCTGATCGATGTGGATCACCAACTGCTACCCGATATCCTGGTCCTGCCG
CTGCTGTGGCTGGGGCTGATCATCAACAGCGCCGACCTGCTGACCGCACTGCCGGACGCACTGTGGGGCGCGGTGATCGG
CTACCTAAGTCTGTGGACAGTGTTCTGGCTGTTCAAACTGGTGACCGGCAAGGACGGCATGGGCCATGGCGACTTCAAGT
TATTGGCGCTGCTTGGCGCCTGGGGCGGCTGGCAGATTCTGCCGATGACGCTACTGATGTCTTCGCTGGTGGGCGTGATC
GTAGGGCTGATTCTGATGCGCTTGCGCAAGGCTGAGATCTCGGCACCGATGCCGTTTGGTCCCTATCTGGCAATTGCCGG
CTGGATTGCCGTGCTCTGGGGTGGTCAAATAACCGACTTCTATTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-124)

Type IV leader peptidase family (Peptidase_A24)

(134-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

54.317

96.528

0.524
  pilD Vibrio campbellii strain DS40M4

51.254

96.875

0.497
  pilD Acinetobacter nosocomialis M2

51.282

94.792

0.486
  pilD Acinetobacter baumannii D1279779

51.282

94.792

0.486
  pilD Neisseria gonorrhoeae MS11

49.259

93.75

0.462