Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P3U62_RS06135 Genome accession   NZ_CP120130
Coordinates   1237677..1238579 (+) Length   300 a.a.
NCBI ID   WP_103323129.1    Uniprot ID   -
Organism   Mammaliicoccus vitulinus strain Dog047     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1232677..1243579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3U62_RS06095 (P3U62_06095) - 1232759..1233313 (+) 555 WP_323707257.1 5-formyltetrahydrofolate cyclo-ligase -
  P3U62_RS06100 (P3U62_06100) - 1233314..1234762 (+) 1449 WP_016911881.1 rhomboid family intramembrane serine protease -
  P3U62_RS06105 (P3U62_06105) - 1234766..1234957 (+) 192 WP_016911882.1 YqgQ family protein -
  P3U62_RS06110 (P3U62_06110) - 1234954..1235925 (+) 972 WP_016911883.1 ROK family glucokinase -
  P3U62_RS06115 (P3U62_06115) - 1235925..1236239 (+) 315 WP_016911884.1 MTH1187 family thiamine-binding protein -
  P3U62_RS06120 (P3U62_06120) - 1236270..1236509 (-) 240 WP_016911885.1 DUF2759 domain-containing protein -
  P3U62_RS06125 (P3U62_06125) - 1236609..1237241 (+) 633 WP_016911886.1 MBL fold metallo-hydrolase -
  P3U62_RS06130 (P3U62_06130) - 1237278..1237523 (-) 246 WP_016911887.1 DUF2626 family protein -
  P3U62_RS06135 (P3U62_06135) comGA 1237677..1238579 (+) 903 WP_103323129.1 competence type IV pilus ATPase ComGA Machinery gene
  P3U62_RS06140 (P3U62_06140) comGB 1238572..1239609 (+) 1038 WP_169034821.1 competence type IV pilus assembly protein ComGB -
  P3U62_RS06145 (P3U62_06145) comGC 1239623..1239940 (+) 318 WP_016911890.1 competence type IV pilus major pilin ComGC Machinery gene
  P3U62_RS06150 (P3U62_06150) - 1239970..1240368 (+) 399 WP_323707258.1 prepilin-type cleavage/methylation domain-containing protein -
  P3U62_RS06155 (P3U62_06155) comGE 1240391..1240633 (+) 243 WP_226908889.1 competence type IV pilus minor pilin ComGE -
  P3U62_RS06160 (P3U62_06160) comGF 1240608..1241042 (+) 435 WP_169034820.1 competence type IV pilus minor pilin ComGF -
  P3U62_RS06165 (P3U62_06165) - 1241277..1241765 (+) 489 WP_016911895.1 shikimate kinase -
  P3U62_RS06170 (P3U62_06170) gcvT 1241958..1243046 (+) 1089 WP_016911896.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34665.86 Da        Isoelectric Point: 6.4127

>NTDB_id=803357 P3U62_RS06135 WP_103323129.1 1237677..1238579(+) (comGA) [Mammaliicoccus vitulinus strain Dog047]
MEKLFKSIIEDAIKSNVSDIHFIPVIDDKIVVQMRFNGDNKDYDLSLDAKLYNRLLIYMKFISHLDVSERNKAQSGQYIY
EHVMNYYLRISTIPISLGIESCAIRITPQYFIESDANRLNSDFIEIRNKMYQSEGLFLFTGPTGSGKTTLMYELMYDLKK
EKNKHIITIEDPVEQTLNGVVQVSVNERANISYQQSFKAILRCDPDVIMIGEIRDEETAKQVIQASLSGHLVLSTMHAKD
NIGAIHRLIELGITVEQIKQGISCIANQRLVKQDESSRCRKMSYMFSEHIIQYMVRDYHE

Nucleotide


Download         Length: 903 bp        

>NTDB_id=803357 P3U62_RS06135 WP_103323129.1 1237677..1238579(+) (comGA) [Mammaliicoccus vitulinus strain Dog047]
ATGGAGAAATTATTTAAATCAATTATCGAAGACGCTATAAAATCAAATGTAAGCGATATTCATTTCATACCTGTAATAGA
CGATAAGATTGTTGTTCAGATGAGATTTAATGGTGACAACAAAGACTATGATTTATCGCTAGATGCTAAATTGTATAACA
GATTGCTAATCTATATGAAATTTATTTCTCACTTAGACGTAAGTGAGAGAAACAAAGCACAAAGTGGTCAGTATATTTAT
GAACATGTTATGAATTATTATTTGAGAATTTCGACGATACCAATTAGTTTAGGGATAGAATCATGTGCAATCAGGATAAC
ACCTCAATATTTTATTGAAAGTGATGCAAATAGGCTTAATTCAGATTTTATAGAAATTAGAAATAAGATGTATCAATCTG
AAGGTTTATTTCTATTTACTGGGCCTACAGGATCTGGTAAGACAACTTTAATGTATGAATTAATGTATGACTTAAAGAAA
GAAAAGAATAAACATATTATTACGATTGAGGATCCTGTGGAACAAACATTAAATGGCGTTGTACAAGTTTCTGTTAATGA
AAGAGCGAATATATCTTATCAACAGTCATTTAAAGCAATTTTAAGATGTGATCCTGATGTTATTATGATAGGTGAAATAA
GAGATGAAGAAACTGCTAAGCAAGTTATACAAGCAAGTTTAAGTGGGCATCTTGTGCTTTCAACTATGCATGCTAAAGAT
AATATCGGTGCAATACATAGATTAATTGAATTAGGCATAACTGTAGAACAAATTAAGCAAGGCATATCGTGTATTGCAAA
TCAGCGATTAGTTAAGCAAGATGAATCAAGTCGCTGTCGAAAAATGAGTTACATGTTTAGTGAACATATCATTCAATATA
TGGTGCGTGATTATCATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

49.832

99

0.493
  comGA Staphylococcus aureus N315

49.832

99

0.493
  comGA Bacillus subtilis subsp. subtilis str. 168

42.238

92.333

0.39