Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   RJ646_RS00310 Genome accession   NZ_CP134358
Coordinates   71517..73598 (-) Length   693 a.a.
NCBI ID   WP_276270042.1    Uniprot ID   -
Organism   Escherichia coli strain TUM13936     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 66517..78598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ646_RS00295 (RJ646_00295) yicH 66636..68345 (-) 1710 WP_059321201.1 AsmA family protein -
  RJ646_RS00300 (RJ646_00300) xanP 68466..69857 (-) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  RJ646_RS00305 (RJ646_00305) gltS 70136..71341 (+) 1206 WP_000468836.1 sodium/glutamate symporter -
  RJ646_RS00310 (RJ646_00310) recG 71517..73598 (-) 2082 WP_276270042.1 ATP-dependent DNA helicase RecG Machinery gene
  RJ646_RS00315 (RJ646_00315) trmH 73604..74293 (-) 690 WP_001070194.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  RJ646_RS00320 (RJ646_00320) spoT 74300..76408 (-) 2109 WP_000280488.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  RJ646_RS00325 (RJ646_00325) rpoZ 76427..76702 (-) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  RJ646_RS00330 (RJ646_00330) gmk 76757..77380 (-) 624 WP_032281852.1 guanylate kinase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76510.47 Da        Isoelectric Point: 7.2083

>NTDB_id=802811 RJ646_RS00310 WP_276270042.1 71517..73598(-) (recG) [Escherichia coli strain TUM13936]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLQTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNITFGGRRM
MTCQISDGSGILTMRFFNFNAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARVVAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACM
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=802811 RJ646_RS00310 WP_276270042.1 71517..73598(-) (recG) [Escherichia coli strain TUM13936]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCGCTCAGTTCCCTAACGGGCGTTGGCGCAGCGCTTAGTAACAAGCTGGCGAA
AATCAACCTGCAGACTGTGCAGGATCTACTCTTACACCTTCCCCTGCGCTACGAAGATCGCACCCATCTCTACCCCATCG
GAGAACTACTACCGGGCGTTTATGCCACGGTGGAAGGCGAAGTGCTGAATTGCAATATCACCTTCGGCGGCCGCCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATTCTCACCATGCGCTTTTTCAACTTCAACGCGGCAATGAAAAATAGCCT
GGCGACGGGCCGCCGCGTGCTGGCCTATGGAGAAGCAAAGCGCGGTAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTGCAGGGCGATCTCAGTACGCCAGAGTTGCAGGAAACGCTCACGCCGGTTTATCCAACAACCGAAGGCGTAAAGCAG
GCCACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACCTGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCGCCGCCGACGCTGCAACTTAGCGATCTGG
AAACCGGGCAGCATCCGGCGCAACGTCGTCTGATTCTGGAAGAACTGCTGGCGCATAACCTCAGCATGTTAGCCTTACGC
GCCGGAGCGCAACGTTTCCATGCCCAGCCGCTGAGCGCCAATGACACGCTGAAAAATAAACTCCTCGCCGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCATGATATGGCGCTGGATGTGCCGATGATGCGTCTGG
TGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGCTGCGTGCGATTGCCCACGGCAAACAGGTG
GCACTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCGA
AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCGCGACTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTCGGAACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGCTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAAGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCGATCCCCCGCACGCTGGCGATGACTGCGTATGCTGATCTCGACACCTCGGTCATTGATGAACTGCCGCCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACTCGCCGTACCGACATCATTGACCGCGTGCGCCACGCCTGCATG
ACTGAAGGTCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCAGAGTTACTGGAAGCGCAGGCGGCGGAAGCTAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAATGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCT
AACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTGCACCAGCTACGTGGGCGTGTAGGTCG
TGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGCGCAAATTCGCCTGCAAGTGCTGC
GCGACAGCAACGACGGGTTTGTGATTGCACAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACTCGTCAG
ACGGGTAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)

DEAD/DEAH box helicase (DEAD)

(292-433)

RecG wedge domain (RecG_wedge)

(15-171)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.381

96.97

0.508
  recG/mmsA Streptococcus pneumoniae R6

38.929

99.711

0.388
  recG/mmsA Streptococcus pneumoniae R36A

38.929

99.711

0.388
  recG Bacillus subtilis subsp. subtilis str. 168

39.362

94.949

0.374