Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RJ647_RS04405 Genome accession   NZ_CP134353
Coordinates   925999..926979 (+) Length   326 a.a.
NCBI ID   WP_086977593.1    Uniprot ID   -
Organism   Escherichia coli strain TUM17668     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 920999..931979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ647_RS04375 (RJ647_04375) - 922246..922551 (+) 306 Protein_863 DUF1202 family protein -
  RJ647_RS04380 (RJ647_04380) hemW 922676..923812 (-) 1137 WP_000239953.1 radical SAM family heme chaperone HemW -
  RJ647_RS04385 (RJ647_04385) rdgB 923805..924398 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  RJ647_RS04390 (RJ647_04390) yggU 924406..924696 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  RJ647_RS04395 (RJ647_04395) yggT 924693..925259 (-) 567 WP_001094820.1 osmotic shock tolerance protein YggT -
  RJ647_RS04400 (RJ647_04400) yggS 925277..925981 (-) 705 WP_000997804.1 pyridoxal phosphate homeostasis protein -
  RJ647_RS04405 (RJ647_04405) pilT 925999..926979 (+) 981 WP_086977593.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RJ647_RS04410 (RJ647_04410) ruvX 927153..927569 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  RJ647_RS04415 (RJ647_04415) yqgE 927569..928132 (-) 564 WP_001301052.1 YqgE/AlgH family protein -
  RJ647_RS04420 (RJ647_04420) gshB 928241..929191 (-) 951 WP_000593260.1 glutathione synthase -
  RJ647_RS04425 (RJ647_04425) rsmE 929204..929935 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RJ647_RS04430 (RJ647_04430) endA 930015..930722 (-) 708 WP_000286492.1 deoxyribonuclease I -
  RJ647_RS04435 (RJ647_04435) yggI 930817..931314 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35985.23 Da        Isoelectric Point: 6.1764

>NTDB_id=802748 RJ647_RS04405 WP_086977593.1 925999..926979(+) (pilT) [Escherichia coli strain TUM17668]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSKNGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEEPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=802748 RJ647_RS04405 WP_086977593.1 925999..926979(+) (pilT) [Escherichia coli strain TUM17668]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCAAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGAACCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365