Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P3U43_RS06690 Genome accession   NZ_CP120062
Coordinates   1344856..1345752 (+) Length   298 a.a.
NCBI ID   WP_017000235.1    Uniprot ID   A0AAP1RT50
Organism   Mammaliicoccus lentus strain Dog127_L     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1339856..1350752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3U43_RS06650 (P3U43_06650) - 1339912..1340466 (+) 555 WP_064204534.1 5-formyltetrahydrofolate cyclo-ligase -
  P3U43_RS06655 (P3U43_06655) - 1340467..1341915 (+) 1449 WP_017000228.1 rhomboid family intramembrane serine protease -
  P3U43_RS06660 (P3U43_06660) - 1341918..1342109 (+) 192 WP_017000229.1 YqgQ family protein -
  P3U43_RS06665 (P3U43_06665) - 1342106..1343077 (+) 972 WP_017000230.1 ROK family glucokinase -
  P3U43_RS06670 (P3U43_06670) - 1343077..1343391 (+) 315 WP_017000231.1 MTH1187 family thiamine-binding protein -
  P3U43_RS06675 (P3U43_06675) - 1343421..1343660 (-) 240 WP_017000232.1 DUF2759 domain-containing protein -
  P3U43_RS06680 (P3U43_06680) - 1343758..1344390 (+) 633 WP_017000233.1 MBL fold metallo-hydrolase -
  P3U43_RS06685 (P3U43_06685) - 1344455..1344703 (-) 249 WP_017000234.1 DUF2626 family protein -
  P3U43_RS06690 (P3U43_06690) comGA 1344856..1345752 (+) 897 WP_017000235.1 competence type IV pilus ATPase ComGA Machinery gene
  P3U43_RS06695 (P3U43_06695) comGB 1345749..1346789 (+) 1041 WP_228064610.1 competence type IV pilus assembly protein ComGB -
  P3U43_RS06700 (P3U43_06700) comGC 1346803..1347120 (+) 318 WP_070045385.1 competence type IV pilus major pilin ComGC Machinery gene
  P3U43_RS06705 (P3U43_06705) comGD 1347120..1347548 (+) 429 WP_017000239.1 competence type IV pilus minor pilin ComGD -
  P3U43_RS06710 (P3U43_06710) comGE 1347532..1347807 (+) 276 WP_017000240.1 competence type IV pilus minor pilin ComGE -
  P3U43_RS06715 (P3U43_06715) comGF 1347821..1348216 (+) 396 WP_320424674.1 competence type IV pilus minor pilin ComGF -
  P3U43_RS06720 (P3U43_06720) - 1348452..1348970 (+) 519 WP_017000243.1 shikimate kinase -
  P3U43_RS06725 (P3U43_06725) gcvT 1349133..1350221 (+) 1089 WP_017000244.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 298 a.a.        Molecular weight: 34435.59 Da        Isoelectric Point: 5.8696

>NTDB_id=802425 P3U43_RS06690 WP_017000235.1 1344856..1345752(+) (comGA) [Mammaliicoccus lentus strain Dog127_L]
METLFKTIIKDAIILKATDIHFIPVKEDRVKVQLRISGENTIYDCDINNALYNRLLIYMKFIAHLDVSERNKAQSGQYIF
DDIYKYYLRISTLPLSLGIESCSIRLTPKYFEEYIDHNIDDNYKIIKEEMFNSEGLFLFTGPTGSGKSTIMYQFLYDLQQ
IKDKHIITIEDPVEQVLDGVVQVSVNEKANINYHNSFKAILRCDPDVVMIGEIRDEETAKQVIQASLSGHLILSTMHAKD
NLGAIYRLLELGISKEQIKQGIACIANQRLLNDQLGNRYRQMTYIFSHEINLYLENKS

Nucleotide


Download         Length: 897 bp        

>NTDB_id=802425 P3U43_RS06690 WP_017000235.1 1344856..1345752(+) (comGA) [Mammaliicoccus lentus strain Dog127_L]
ATGGAAACTTTATTTAAGACAATTATCAAAGATGCTATTATTTTGAAAGCAACTGACATTCATTTTATTCCAGTGAAAGA
GGATCGGGTCAAAGTTCAGTTGAGGATAAGTGGTGAGAATACGATTTATGATTGTGATATTAATAACGCTTTATATAATC
GTTTATTAATTTATATGAAGTTTATTGCTCATCTAGATGTAAGTGAAAGGAATAAAGCTCAAAGTGGTCAATATATATTT
GATGATATATATAAATATTATTTAAGAATATCCACTTTACCTTTAAGTCTAGGAATTGAGTCTTGCTCAATTAGGTTAAC
GCCTAAATATTTCGAAGAATATATTGACCATAATATTGATGACAATTATAAAATAATTAAAGAAGAAATGTTTAATTCCG
AAGGCTTATTTTTGTTCACAGGACCAACCGGTTCGGGTAAATCGACGATTATGTACCAATTTTTATATGACTTACAACAA
ATTAAAGATAAACATATTATTACGATTGAAGACCCAGTTGAACAAGTTTTAGATGGTGTAGTACAAGTTTCTGTAAATGA
AAAAGCAAACATAAATTATCATAATTCTTTTAAAGCCATTTTAAGATGTGATCCAGATGTTGTCATGATAGGAGAAATTA
GAGATGAAGAAACAGCCAAGCAAGTTATACAAGCAAGTTTAAGTGGTCATTTAATATTATCTACAATGCATGCGAAAGAT
AATTTAGGGGCTATTTATAGACTTTTAGAACTTGGAATATCTAAAGAGCAAATAAAACAAGGTATCGCATGTATAGCCAA
TCAAAGACTTTTAAATGACCAATTAGGAAATAGATATAGACAAATGACTTATATATTTAGTCATGAAATTAATCTATATT
TAGAAAATAAATCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

50.993

100

0.517
  comGA Staphylococcus aureus N315

50.993

100

0.517
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

39.068

93.624

0.366