Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   P3K50_RS07405 Genome accession   NZ_CP119715
Coordinates   1567595..1568092 (-) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain CUVET17-649     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1562595..1573092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3K50_RS07380 (P3K50_07365) gcvPB 1562757..1564229 (-) 1473 WP_000202179.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  P3K50_RS07385 (P3K50_07370) gcvPA 1564222..1565568 (-) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  P3K50_RS07390 (P3K50_07375) gcvT 1565588..1566679 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  P3K50_RS07395 (P3K50_07380) - 1566838..1567362 (-) 525 WP_001015120.1 shikimate kinase -
  P3K50_RS07400 (P3K50_07385) - 1567352..1567498 (-) 147 WP_001789879.1 hypothetical protein -
  P3K50_RS07405 (P3K50_07390) comGF 1567595..1568092 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  P3K50_RS07410 (P3K50_07395) comGE 1568010..1568309 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  P3K50_RS07415 (P3K50_07400) comGD 1568296..1568742 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  P3K50_RS07420 (P3K50_07405) comGC 1568720..1569031 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  P3K50_RS07425 (P3K50_07410) - 1569149..1570321 (-) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  P3K50_RS07430 (P3K50_07415) comGB 1570407..1571447 (-) 1041 WP_404969097.1 competence type IV pilus assembly protein ComGB Machinery gene
  P3K50_RS07435 (P3K50_07420) comGA 1571419..1572393 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  P3K50_RS07440 (P3K50_07425) - 1572445..1573068 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=800782 P3K50_RS07405 WP_001825340.1 1567595..1568092(-) (comGF) [Staphylococcus aureus strain CUVET17-649]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=800782 P3K50_RS07405 WP_001825340.1 1567595..1568092(-) (comGF) [Staphylococcus aureus strain CUVET17-649]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97