Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RG644_RS07405 Genome accession   NZ_CP133751
Coordinates   1573100..1573723 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain ZYPA1927     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1568100..1578723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG644_RS07390 (RG644_07390) - 1568787..1571258 (-) 2472 WP_031756300.1 penicillin-binding protein 1A -
  RG644_RS07395 (RG644_07395) pilM 1571443..1572507 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  RG644_RS07400 (RG644_07400) pilN 1572507..1573103 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  RG644_RS07405 (RG644_07405) pilO 1573100..1573723 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  RG644_RS07410 (RG644_07410) pilP 1573720..1574244 (+) 525 WP_223111084.1 type 4a pilus biogenesis lipoprotein PilP -
  RG644_RS07415 (RG644_07415) pilQ 1574298..1576442 (+) 2145 WP_003123619.1 type 4a pilus secretin PilQ Machinery gene
  RG644_RS07420 (RG644_07420) aroK 1576454..1576972 (+) 519 WP_003095833.1 shikimate kinase AroK -
  RG644_RS07425 (RG644_07425) aroB 1577022..1578128 (+) 1107 WP_003107839.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=800689 RG644_RS07405 WP_003103268.1 1573100..1573723(+) (pilO) [Pseudomonas aeruginosa strain ZYPA1927]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=800689 RG644_RS07405 WP_003103268.1 1573100..1573723(+) (pilO) [Pseudomonas aeruginosa strain ZYPA1927]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401