Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RG462_RS30230 Genome accession   NZ_CP133737
Coordinates   6387355..6387978 (-) Length   207 a.a.
NCBI ID   WP_003095838.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain FI-14/157     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6382355..6392978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG462_RS30210 (RG462_30210) aroB 6382950..6384056 (-) 1107 WP_003095831.1 3-dehydroquinate synthase -
  RG462_RS30215 (RG462_30215) aroK 6384106..6384624 (-) 519 WP_003095833.1 shikimate kinase AroK -
  RG462_RS30220 (RG462_30220) pilQ 6384636..6386780 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  RG462_RS30225 (RG462_30225) comL/pilP 6386834..6387358 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  RG462_RS30230 (RG462_30230) pilO 6387355..6387978 (-) 624 WP_003095838.1 type 4a pilus biogenesis protein PilO Machinery gene
  RG462_RS30235 (RG462_30235) pilN 6387975..6388571 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  RG462_RS30240 (RG462_30240) pilM 6388571..6389635 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  RG462_RS30245 (RG462_30245) - 6389820..6392291 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22805.23 Da        Isoelectric Point: 4.7787

>NTDB_id=800534 RG462_RS30230 WP_003095838.1 6387355..6387978(-) (pilO) [Pseudomonas aeruginosa strain FI-14/157]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=800534 RG462_RS30230 WP_003095838.1 6387355..6387978(-) (pilO) [Pseudomonas aeruginosa strain FI-14/157]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGGTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

44.211

91.787

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.684

91.787

0.401