Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   RFN65_RS12305 Genome accession   NZ_CP133703
Coordinates   2420723..2422669 (-) Length   648 a.a.
NCBI ID   WP_014476782.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 2415723..2427669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS12275 (RFN65_12275) yloU 2417384..2417746 (-) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  RFN65_RS12280 (RFN65_12280) rpmB 2418023..2418211 (+) 189 WP_003221548.1 50S ribosomal protein L28 -
  RFN65_RS12285 (RFN65_12285) spoVM 2418284..2418364 (-) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  RFN65_RS12290 (RFN65_12290) thiN 2418437..2419081 (-) 645 WP_014479765.1 thiamine diphosphokinase -
  RFN65_RS12295 (RFN65_12295) rpe 2419154..2419807 (-) 654 WP_063335342.1 ribulose-phosphate 3-epimerase -
  RFN65_RS12300 (RFN65_12300) rsgA 2419812..2420708 (-) 897 WP_063335341.1 ribosome small subunit-dependent GTPase A -
  RFN65_RS12305 (RFN65_12305) stkP 2420723..2422669 (-) 1947 WP_014476782.1 serine/threonine protein kinase PrkC Regulator
  RFN65_RS12310 (RFN65_12310) prpC 2422663..2423427 (-) 765 WP_015383633.1 protein-serine/threonine phosphatase PrpC -
  RFN65_RS12315 (RFN65_12315) rlmN 2423434..2424525 (-) 1092 WP_014476780.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  RFN65_RS12320 (RFN65_12320) rsmB 2424529..2425872 (-) 1344 WP_015383632.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  RFN65_RS12325 (RFN65_12325) fmt 2425859..2426812 (-) 954 WP_063335337.1 methionyl-tRNA formyltransferase -
  RFN65_RS12330 (RFN65_12330) def 2426817..2427299 (-) 483 WP_015383630.1 peptide deformylase -

Sequence

Protein


Download         Length: 648 a.a.        Molecular weight: 71910.59 Da        Isoelectric Point: 4.7871

>NTDB_id=800152 RFN65_RS12305 WP_014476782.1 2420723..2422669(-) (stkP) [Bacillus subtilis strain CP35]
MLIGKRISGRYQILRVIGGGGMANVYLAEDIILDREVAIKILRFDYANDNEFIRRFRREAQSASSLDHPNIVSIYDLGEE
DDIYYIVMEYVEGMTLKEYITANGPLHPKEALNIMEQIVSAIAHAHQNQIVHRDIKPHNILIDHMGNIKVTDFGIATALS
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGRIPFDGESAVSIALKHLQAETPSTKRWNPSVPQS
VENIILKATAKDPFHRYETAEDMEADIKTAFDADRLNEKRFTIQEDEEMTKAIPIIKDEELAKAAGEKEAEVTTAQENKT
KKNGKRKKWPWVLLTICLVFITAGILAVTVFPSLFMPKDVKIPDVSGMEYEKAAGLLEKEGLQVDSEVLEISDEKIEEGL
MVKTDPKADTTVKEGATVTLYKSTGKAKTEIGDVTGQTVDQAKKALKDQGFKHVTVNEVNDEKNAGTVIDQNPSAGTELV
PSEDQVKLTVSIGPEDITLRDLKTYSKEAASGYLEDNGLKLVEKEAYSDDVPEGQVVKQKPAAGTAVKPGNEVEVTFSLG
PEKKPAKTVKEKVKIPYEPENEGDELQVQIAVDDADHSISDTYEEFKIKEPTERTIELKIEPGQKGYYQVMVNNKVVSYK
TIEYPKDE

Nucleotide


Download         Length: 1947 bp        

>NTDB_id=800152 RFN65_RS12305 WP_014476782.1 2420723..2422669(-) (stkP) [Bacillus subtilis strain CP35]
GTGCTAATCGGCAAGCGGATCAGCGGGCGTTACCAGATTCTCCGCGTTATAGGCGGCGGGGGAATGGCCAACGTGTATTT
AGCTGAGGATATCATTCTAGACCGTGAAGTCGCAATTAAAATCCTGCGGTTTGACTATGCAAATGACAATGAATTTATCA
GACGTTTCCGCAGAGAAGCCCAATCCGCATCAAGCCTCGATCACCCGAATATTGTCAGCATTTACGATTTGGGCGAGGAA
GATGATATTTATTATATTGTCATGGAATACGTTGAAGGCATGACGCTTAAAGAATACATAACAGCAAATGGGCCGCTTCA
CCCTAAAGAAGCGCTGAACATCATGGAGCAAATTGTCTCAGCCATCGCTCATGCCCATCAAAACCAGATTGTTCACAGAG
ACATCAAGCCGCATAACATTTTGATTGATCATATGGGAAATATCAAAGTAACGGATTTTGGAATTGCGACGGCACTAAGT
TCGACCACAATCACCCATACCAATTCAGTTCTGGGCTCGGTCCATTACTTATCACCTGAACAGGCCCGGGGCGGCTTAGC
CACAAAAAAATCGGATATTTATGCGCTTGGAATCGTTCTATTTGAGCTTTTAACCGGCCGTATTCCGTTTGATGGAGAGT
CAGCAGTCAGCATCGCCTTGAAGCATCTTCAAGCGGAAACTCCTTCGACAAAAAGGTGGAATCCATCGGTCCCCCAAAGC
GTTGAAAACATCATACTCAAGGCAACTGCCAAAGATCCTTTTCATCGCTACGAAACGGCTGAAGACATGGAAGCAGACAT
AAAAACAGCTTTTGATGCCGACAGACTCAATGAAAAGAGATTTACGATTCAAGAAGATGAAGAAATGACAAAAGCGATAC
CTATCATTAAAGATGAAGAACTCGCTAAAGCTGCTGGCGAAAAAGAAGCTGAAGTGACAACCGCACAAGAAAACAAAACA
AAGAAGAACGGCAAAAGAAAAAAGTGGCCGTGGGTTTTGCTCACGATATGCCTCGTTTTTATCACAGCTGGAATTCTTGC
GGTCACTGTTTTTCCATCGCTTTTCATGCCTAAGGATGTCAAAATACCTGATGTCTCCGGAATGGAATACGAAAAAGCCG
CAGGGCTCTTGGAAAAAGAAGGTTTACAGGTTGATTCCGAGGTGTTGGAAATCTCAGATGAAAAAATTGAAGAGGGCCTG
ATGGTAAAAACGGACCCTAAAGCGGATACCACAGTCAAAGAAGGCGCCACGGTCACCCTTTATAAGAGCACCGGAAAAGC
AAAAACGGAGATCGGTGATGTGACAGGCCAAACGGTCGACCAGGCAAAAAAAGCGTTGAAGGACCAAGGGTTTAAACATG
TAACGGTAAATGAAGTGAATGACGAGAAAAATGCGGGCACTGTCATTGACCAAAATCCTTCAGCAGGGACTGAGCTTGTC
CCGAGTGAAGATCAAGTCAAACTTACAGTCAGTATCGGACCCGAAGACATTACGCTTAGAGACTTGAAAACCTACAGTAA
AGAAGCAGCGTCTGGATATCTGGAAGACAACGGATTGAAGCTTGTAGAAAAAGAAGCATACTCAGATGATGTTCCAGAAG
GACAGGTTGTCAAACAAAAACCAGCAGCAGGTACGGCAGTAAAGCCGGGAAACGAAGTTGAAGTGACATTCTCTCTCGGA
CCAGAGAAAAAACCTGCGAAAACAGTGAAAGAAAAGGTCAAGATCCCCTACGAACCAGAAAATGAAGGGGACGAGCTTCA
AGTGCAAATCGCGGTTGACGATGCGGATCACAGCATCTCTGACACTTACGAAGAATTTAAGATAAAAGAGCCGACTGAAC
GAACGATCGAACTAAAGATTGAACCCGGCCAAAAAGGGTACTATCAAGTAATGGTAAACAATAAAGTTGTCAGCTACAAA
ACCATTGAGTATCCGAAAGATGAATAA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(11-261)

PASTA domain (PASTA)

(360-423)

PASTA domain (PASTA)

(498-558)

PASTA domain (PASTA)

(430-491)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

40.517

89.506

0.363
  stkP Streptococcus pneumoniae D39

40.345

89.506

0.361
  stkP Streptococcus pneumoniae R6

40.345

89.506

0.361