Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   RFN65_RS12255 Genome accession   NZ_CP133703
Coordinates   2411953..2414001 (-) Length   682 a.a.
NCBI ID   WP_309529604.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2406953..2419001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS12225 (RFN65_12225) rncS 2407352..2408101 (-) 750 WP_003232030.1 ribonuclease III -
  RFN65_RS12230 (RFN65_12230) acpP 2408241..2408474 (-) 234 WP_003154310.1 acyl carrier protein -
  RFN65_RS12235 (RFN65_12235) fabG 2408558..2409298 (-) 741 WP_003232035.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  RFN65_RS12240 (RFN65_12240) fabD 2409291..2410244 (-) 954 WP_003245314.1 ACP S-malonyltransferase -
  RFN65_RS12245 (RFN65_12245) plsX 2410263..2411264 (-) 1002 WP_003232041.1 phosphate acyltransferase PlsX -
  RFN65_RS12250 (RFN65_12250) fapR 2411278..2411844 (-) 567 WP_003221561.1 transcription factor FapR -
  RFN65_RS12255 (RFN65_12255) recG 2411953..2414001 (-) 2049 WP_309529604.1 ATP-dependent DNA helicase RecG Machinery gene
  RFN65_RS12260 (RFN65_12260) sdaAA 2413979..2414881 (-) 903 WP_003232049.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  RFN65_RS12265 (RFN65_12265) sdaAB 2414906..2415568 (-) 663 WP_003232050.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  RFN65_RS12270 (RFN65_12270) fakA 2415707..2417368 (-) 1662 WP_015483218.1 DAK2 domain-containing protein -
  RFN65_RS12275 (RFN65_12275) yloU 2417384..2417746 (-) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  RFN65_RS12280 (RFN65_12280) rpmB 2418023..2418211 (+) 189 WP_003221548.1 50S ribosomal protein L28 -
  RFN65_RS12285 (RFN65_12285) spoVM 2418284..2418364 (-) 81 WP_003221545.1 stage V sporulation protein SpoVM -

Sequence

Protein


Download         Length: 682 a.a.        Molecular weight: 78114.69 Da        Isoelectric Point: 7.4462

>NTDB_id=800151 RFN65_RS12255 WP_309529604.1 2411953..2414001(-) (recG) [Bacillus subtilis strain CP35]
MKQHQQTSIANIKGIGPETEKTLHELGINDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Nucleotide


Download         Length: 2049 bp        

>NTDB_id=800151 RFN65_RS12255 WP_309529604.1 2411953..2414001(-) (recG) [Bacillus subtilis strain CP35]
GTGAAACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTACACGAACTCGG
TATTAATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGCTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTCGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATCACTGCCGTATGTTTTAACCGGCCCTATTTGAAGAAGAAGCTTTC
GCTTGGCTCTGTGGTGACGGTTTCAGGTAAATGGGATAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGACAAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGCTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCGCTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAAGCGTTAAAAGCAATGCACCAGCCTGAAACAAGGGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAGCGT
TTTTCAAACGAAGAACTCATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAACCGTCTTCTTCAAGGGGACGTTGGATCAGGAAAAACGGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCTGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGGAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCACGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGCTGAGTCTCGTTATTACTGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGGCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACATTAGCGATCACAGTGTTCGGTGAAAT
GGATGTATCTGTCATTGATGAGATGCCGGCTGGACGAAAGCGAATTGAAACCTATTGGGTAAAACATGACATGCTGGATC
GTATTTTGGCATTTGTCGAAAAAGAATTAAAGCAAGGCAGACAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAC
AAGCTTGATGTGCAAAACGCCATTGACGTATACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGGAAGCTACATTCCGATGAAAAAGACCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CTACTGTTGTTGAGGTTGGCGTGAATGTTCCGAATGCAACGATTATGGTGATTTATGACGCCGACCGTTTCGGACTATCA
CAGCTTCACCAGCTGCGTGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAGACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGAAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(260-415)

Helicase conserved C-terminal domain (Helicase_C)

(452-572)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)

RecG wedge domain (RecG_wedge)

(9-165)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Bacillus subtilis subsp. subtilis str. 168

99.707

100

0.997
  recG/mmsA Streptococcus pneumoniae R6

48.968

99.413

0.487
  recG/mmsA Streptococcus pneumoniae R36A

48.968

99.413

0.487
  recG Neisseria meningitidis strain C311

39.542

96.041

0.38