Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PYW35_RS06225 Genome accession   NZ_CP118974
Coordinates   1229136..1230038 (+) Length   300 a.a.
NCBI ID   WP_103323129.1    Uniprot ID   -
Organism   Mammaliicoccus vitulinus strain DSM 15615     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1224136..1235038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW35_RS06185 - 1224219..1224773 (+) 555 WP_103323127.1 5-formyltetrahydrofolate cyclo-ligase -
  PYW35_RS06190 - 1224774..1226222 (+) 1449 WP_103323128.1 rhomboid family intramembrane serine protease -
  PYW35_RS06195 - 1226226..1226417 (+) 192 WP_016911882.1 YqgQ family protein -
  PYW35_RS06200 - 1226414..1227385 (+) 972 WP_016911883.1 ROK family glucokinase -
  PYW35_RS06205 - 1227385..1227699 (+) 315 WP_016911884.1 MTH1187 family thiamine-binding protein -
  PYW35_RS06210 - 1227729..1227968 (-) 240 WP_016911885.1 DUF2759 domain-containing protein -
  PYW35_RS06215 - 1228068..1228700 (+) 633 WP_016911886.1 MBL fold metallo-hydrolase -
  PYW35_RS06220 - 1228737..1228982 (-) 246 WP_016911887.1 DUF2626 family protein -
  PYW35_RS06225 comGA 1229136..1230038 (+) 903 WP_103323129.1 competence type IV pilus ATPase ComGA Machinery gene
  PYW35_RS06230 comGB 1230031..1231068 (+) 1038 WP_204107810.1 competence type IV pilus assembly protein ComGB -
  PYW35_RS06235 comGC 1231082..1231399 (+) 318 WP_016911890.1 competence type IV pilus major pilin ComGC Machinery gene
  PYW35_RS06240 comGD 1231399..1231827 (+) 429 WP_103323612.1 competence type IV pilus minor pilin ComGD -
  PYW35_RS06245 comGE 1231811..1232092 (+) 282 WP_016911892.1 competence type IV pilus minor pilin ComGE -
  PYW35_RS06250 comGF 1232067..1232501 (+) 435 WP_204107811.1 competence type IV pilus minor pilin ComGF -
  PYW35_RS06255 - 1232736..1233224 (+) 489 WP_103323613.1 shikimate kinase -
  PYW35_RS06260 gcvT 1233417..1234505 (+) 1089 WP_103323614.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34665.86 Da        Isoelectric Point: 6.4127

>NTDB_id=797045 PYW35_RS06225 WP_103323129.1 1229136..1230038(+) (comGA) [Mammaliicoccus vitulinus strain DSM 15615]
MEKLFKSIIEDAIKSNVSDIHFIPVIDDKIVVQMRFNGDNKDYDLSLDAKLYNRLLIYMKFISHLDVSERNKAQSGQYIY
EHVMNYYLRISTIPISLGIESCAIRITPQYFIESDANRLNSDFIEIRNKMYQSEGLFLFTGPTGSGKTTLMYELMYDLKK
EKNKHIITIEDPVEQTLNGVVQVSVNERANISYQQSFKAILRCDPDVIMIGEIRDEETAKQVIQASLSGHLVLSTMHAKD
NIGAIHRLIELGITVEQIKQGISCIANQRLVKQDESSRCRKMSYMFSEHIIQYMVRDYHE

Nucleotide


Download         Length: 903 bp        

>NTDB_id=797045 PYW35_RS06225 WP_103323129.1 1229136..1230038(+) (comGA) [Mammaliicoccus vitulinus strain DSM 15615]
ATGGAGAAATTATTTAAATCAATTATCGAAGACGCTATAAAATCAAATGTAAGCGATATTCATTTCATACCTGTAATAGA
CGATAAGATTGTTGTTCAGATGAGATTTAATGGTGACAACAAAGACTATGATTTATCGCTAGATGCTAAATTGTATAACA
GATTGCTAATCTATATGAAATTTATTTCTCACTTAGACGTAAGTGAGAGAAACAAAGCACAAAGTGGTCAGTATATTTAT
GAACATGTTATGAATTATTATTTAAGAATTTCGACGATACCAATTAGTTTAGGGATAGAATCATGTGCAATCAGGATAAC
ACCTCAATATTTTATTGAAAGTGATGCAAATAGGCTTAATTCAGATTTTATAGAAATTAGAAATAAGATGTATCAATCTG
AAGGTTTATTTCTATTTACTGGGCCTACAGGATCTGGTAAGACAACTTTAATGTATGAATTAATGTATGACTTAAAGAAA
GAAAAGAATAAACATATTATTACGATTGAGGATCCTGTGGAACAAACATTAAATGGCGTTGTACAAGTTTCTGTTAATGA
AAGAGCGAATATATCTTATCAACAGTCATTTAAAGCAATTTTAAGATGTGATCCTGATGTTATTATGATAGGTGAAATAA
GAGATGAAGAAACTGCTAAGCAAGTTATACAAGCAAGTTTAAGTGGGCATCTTGTGCTTTCAACTATGCATGCTAAAGAT
AATATCGGTGCAATACATAGATTAATTGAATTAGGCATAACTGTAGAACAAATTAAGCAAGGCATATCGTGTATTGCAAA
TCAGCGATTAGTTAAGCAAGATGAATCAAGTCGCTGTCGAAAAATGAGTTACATGTTTAGTGAACATATCATTCAATATA
TGGTGCGTGATTATCATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

49.832

99

0.493
  comGA Staphylococcus aureus N315

49.832

99

0.493
  comGA Bacillus subtilis subsp. subtilis str. 168

42.238

92.333

0.39