Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   PYW37_RS05535 Genome accession   NZ_CP118969
Coordinates   1076252..1077601 (-) Length   449 a.a.
NCBI ID   WP_025017173.1    Uniprot ID   A0A0B8R081
Organism   Lactococcus lactis strain DSM 20481     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1071252..1082601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW37_RS05505 - 1071381..1072913 (+) 1533 WP_023189345.1 Nramp family divalent metal transporter -
  PYW37_RS05510 - 1073015..1073983 (+) 969 WP_003130649.1 PhoH family protein -
  PYW37_RS05515 - 1074041..1074520 (+) 480 WP_003130650.1 NUDIX hydrolase -
  PYW37_RS05520 ybeY 1074672..1075160 (+) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  PYW37_RS05525 - 1075144..1075599 (+) 456 WP_003130653.1 diacylglycerol kinase -
  PYW37_RS05530 - 1075613..1076191 (+) 579 WP_023189343.1 nucleotidyltransferase family protein -
  PYW37_RS05535 comFA 1076252..1077601 (-) 1350 WP_025017173.1 DEAD/DEAH box helicase Machinery gene
  PYW37_RS05540 - 1077658..1078287 (+) 630 WP_003130660.1 YigZ family protein -
  PYW37_RS05545 - 1078421..1078918 (+) 498 WP_023189340.1 VanZ family protein -
  PYW37_RS05550 - 1078966..1079985 (+) 1020 WP_012897709.1 DUF475 domain-containing protein -
  PYW37_RS05555 - 1080100..1080846 (+) 747 WP_023189339.1 bifunctional metallophosphatase/5'-nucleotidase -
  PYW37_RS05560 glyQ 1081132..1082085 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51491.09 Da        Isoelectric Point: 10.1773

>NTDB_id=796956 PYW37_RS05535 WP_025017173.1 1076252..1077601(-) (comFA) [Lactococcus lactis strain DSM 20481]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPNLGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDNYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKITSVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKNRYHLISRITNSKRSWCS

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=796956 PYW37_RS05535 WP_025017173.1 1076252..1077601(-) (comFA) [Lactococcus lactis strain DSM 20481]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAAATTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAACTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTACTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACCATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAACAGGTACCACCTTATATCACGCATCACAAATTCTAAAAGAAGCTGGTGTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B8R081

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.74

85.523

0.639
  comFA/cflA Streptococcus pneumoniae Rx1

52.308

86.86

0.454
  comFA/cflA Streptococcus pneumoniae D39

52.308

86.86

0.454
  comFA/cflA Streptococcus pneumoniae R6

52.308

86.86

0.454
  comFA/cflA Streptococcus pneumoniae TIGR4

52.308

86.86

0.454
  comFA/cflA Streptococcus mitis SK321

52.174

87.082

0.454
  comFA/cflA Streptococcus mitis NCTC 12261

51.407

87.082

0.448
  comFA Bacillus subtilis subsp. subtilis str. 168

36.203

100

0.365