Detailed information NTG0796690

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: PYW30_RS04195
• Genome accession: NZ_CP118950
• Coordinates: 836097..837353 (-)
• Length: 418 a.a.
• NCBI ID: WP_042218689.1
• Uniprot ID: -
• Organism: Lactococcus garvieae subsp. garvieae strain DSM 20684
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 831097..842353

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PYW30_RS04160	rpmA	831217..831501 (+)	285	WP_004257956.1	50S ribosomal protein L27	-
PYW30_RS04165	ald	831852..832958 (+)	1107	WP_042218682.1	alanine dehydrogenase	-
PYW30_RS04170	-	833076..834035 (+)	960	WP_004257953.1	PhoH family protein	-
PYW30_RS04175	-	834037..834510 (+)	474	WP_004257950.1	NUDIX hydrolase	-
PYW30_RS04180	ybeY	834520..835008 (+)	489	WP_042218684.1	rRNA maturation RNase YbeY	-
PYW30_RS04185	-	834992..835453 (+)	462	WP_042218685.1	diacylglycerol kinase family protein	-
PYW30_RS04190	-	835456..836100 (-)	645	WP_042218687.1	ComF family protein	-
PYW30_RS04195	comFA	836097..837353 (-)	1257	WP_042218689.1	DEAD/DEAH box helicase	Machinery gene
PYW30_RS04200	-	837408..838031 (+)	624	WP_042218691.1	YigZ family protein	-
PYW30_RS04205	-	838045..839067 (+)	1023	WP_023889525.1	DUF475 domain-containing protein	-
PYW30_RS04210	-	839097..840119 (-)	1023	WP_042218693.1	alpha/beta hydrolase	-
PYW30_RS04215	-	840438..841217 (+)	780	WP_019292766.1	formate/nitrite transporter family protein	-
PYW30_RS04220	glyQ	841231..842199 (+)	969	WP_004257927.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 47919.74 Da
• Isoelectric Point: 10.0108

>NTDB_id=796690 PYW30_RS04195 WP_042218689.1 836097..837353(-) (comFA) [Lactococcus garvieae subsp. garvieae strain DSM 20684]
MDELYGRLLLQKELTKLPDKATLFDGMQDVSKTVMMCNRCGKKIKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQEN
FSAASFLNWQGKLTASQQHVSDSLVKLHQQQKTVLVQAVTGAGKTEMIYQSIDNTLKKGKAVGLTSPRIDVCLELYHRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLLILDEVDAFPFPDNEMLYFALTQARKPSSSLIYLTATTTDN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRQGEKLCQDLQSNFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHYNFTRSSLIQIAGRVGRSTERPEGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFL

Nucleotide

• Length: 1257 bp

>NTDB_id=796690 PYW30_RS04195 WP_042218689.1 836097..837353(-) (comFA) [Lactococcus garvieae subsp. garvieae strain DSM 20684]
ATGGATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGACGAAACTTCCAGATAAAGCAACACTTTTTGATGGGAT
GCAAGATGTTTCCAAAACAGTGATGATGTGCAACCGCTGTGGCAAAAAGATCAAGAAAAAAGAGGTCCTTCTCCCTGTTG
GTGCTTATTATTGTCCTCATTGTATTCAAATGGGACGTGTTCGCTCGGATGAAAAACTTTACCACCTTCCACAGGAGAAT
TTTTCAGCAGCTTCTTTTCTCAACTGGCAGGGAAAACTCACAGCATCTCAGCAACATGTATCCGATAGCCTAGTCAAATT
GCATCAGCAACAAAAAACAGTACTGGTACAGGCGGTTACTGGTGCAGGAAAAACCGAAATGATTTATCAGAGCATTGATA
ACACTCTTAAAAAAGGAAAAGCTGTTGGTTTAACTAGCCCGCGTATTGATGTCTGTCTAGAGCTCTATCATCGCTTAAAG
AGAGATTTTTCTTGTCCCATCTCGCTTTTGCACGGAAAGAGTGAAAAATACAGCCGCTCACCACTCGTAATTGCAACGAC
ACATCAGCTTATGCGTTTTCGCCATGCCTTTGACTTACTCATTCTTGATGAGGTTGATGCCTTTCCCTTTCCAGACAATG
AAATGCTTTATTTTGCGCTGACTCAAGCCCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACCACCACCGATAAT
TTGGAAAAACAAGTCAAATTAGGACAAATCGAGAAGCTACAGCTTCCACGACGTTTTCATGGTTTTCCTCTGGTACTCCC
GCAATTTTTTTGGCAAAGCAAGTTTTACAAAATGGTCAAAAAACAACGCGAATCTGGTTTTCCCTTACTTATCTTTGTTC
CTGAAATAAGACAGGGAGAAAAACTCTGCCAAGATTTACAGAGTAATTTTCCTCATGAAGAAATTGCTTTTGTAGCGTCA
ACAAGTCTTGAACGCTTAGAGGCTGTGGAACGCTTTCGTCAGGGTAATATTTCTATCCTTGTCTCAACGACCATTTTAGA
ACGTGGTGTAACTTTCCCTAAGGTTGACGTTTTTGTGTTTCAAAGTCACCACTATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGAGGGTAAGGTATTTTTCTTTCATCTAGGAAAAACAACAGCG
ATGTTGGAAGCCTATAAGAATATCAGAAATATGAATAAAGCAGGAGGGTTCCTATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (92-236)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	57.398	93.78	0.538
comFA/cflA	Streptococcus mitis SK321	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae TIGR4	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae Rx1	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae D39	52.113	100	0.531
comFA/cflA	Streptococcus pneumoniae R6	52.113	100	0.531
comFA/cflA	Streptococcus mitis NCTC 12261	51.174	100	0.522
comFA	Bacillus subtilis subsp. subtilis str. 168	38.861	96.651	0.376
comFA	Latilactobacillus sakei subsp. sakei 23K	38.539	94.976	0.366