Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PXG99_RS00810 Genome accession   NZ_CP118911
Coordinates   158180..158794 (+) Length   204 a.a.
NCBI ID   WP_029973402.1    Uniprot ID   -
Organism   Bacillus velezensis strain XRD006     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 153180..163794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PXG99_RS00775 (PXG99_00775) aroE 153867..154709 (+) 843 WP_003152856.1 shikimate dehydrogenase -
  PXG99_RS00780 (PXG99_00780) yhbY 154703..154993 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  PXG99_RS00785 (PXG99_00785) - 155004..155573 (+) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  PXG99_RS00790 (PXG99_00790) yqeK 155563..156123 (+) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PXG99_RS00795 (PXG99_00795) rsfS 156141..156497 (+) 357 WP_007408260.1 ribosome silencing factor -
  PXG99_RS00800 (PXG99_00800) - 156494..157231 (+) 738 WP_015417856.1 class I SAM-dependent methyltransferase -
  PXG99_RS00805 (PXG99_00805) comER 157300..158121 (-) 822 WP_029973401.1 late competence protein ComER -
  PXG99_RS00810 (PXG99_00810) comEA 158180..158794 (+) 615 WP_029973402.1 helix-hairpin-helix domain-containing protein Machinery gene
  PXG99_RS00815 (PXG99_00815) - 158861..159430 (+) 570 WP_003152868.1 ComE operon protein 2 -
  PXG99_RS00820 (PXG99_00820) comEC 159431..161782 (+) 2352 WP_029973403.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PXG99_RS00825 (PXG99_00825) - 161801..161935 (-) 135 WP_003152870.1 YqzM family protein -
  PXG99_RS00830 (PXG99_00830) - 161976..162127 (+) 152 Protein_164 hypothetical protein -
  PXG99_RS00835 (PXG99_00835) holA 162167..163262 (+) 1096 Protein_165 DNA polymerase III subunit delta -
  PXG99_RS00840 (PXG99_00840) rpsT 163225..163491 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22240.29 Da        Isoelectric Point: 5.0355

>NTDB_id=796211 PXG99_RS00810 WP_029973402.1 158180..158794(+) (comEA) [Bacillus velezensis strain XRD006]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=796211 PXG99_RS00810 WP_029973402.1 158180..158794(+) (comEA) [Bacillus velezensis strain XRD006]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363