Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PYH69_RS07865 Genome accession   NZ_CP118848
Coordinates   1587754..1588650 (-) Length   298 a.a.
NCBI ID   WP_017000235.1    Uniprot ID   A0AAP1RT50
Organism   Mammaliicoccus lentus strain 7048     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1582754..1593650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYH69_RS07830 (PYH69_07830) gcvT 1583285..1584373 (-) 1089 WP_017000244.1 glycine cleavage system aminomethyltransferase GcvT -
  PYH69_RS07835 (PYH69_07835) - 1584536..1585054 (-) 519 WP_017000243.1 shikimate kinase -
  PYH69_RS07840 (PYH69_07840) comGF 1585290..1585685 (-) 396 WP_237687866.1 competence type IV pilus minor pilin ComGF -
  PYH69_RS07845 (PYH69_07845) comGE 1585699..1585974 (-) 276 WP_017000240.1 competence type IV pilus minor pilin ComGE -
  PYH69_RS07850 (PYH69_07850) comGD 1585958..1586386 (-) 429 WP_017000239.1 competence type IV pilus minor pilin ComGD -
  PYH69_RS07855 (PYH69_07855) comGC 1586386..1586703 (-) 318 WP_070045385.1 competence type IV pilus major pilin ComGC Machinery gene
  PYH69_RS07860 (PYH69_07860) comGB 1586717..1587754 (-) 1038 WP_064211062.1 competence type IV pilus assembly protein ComGB -
  PYH69_RS07865 (PYH69_07865) comGA 1587754..1588650 (-) 897 WP_017000235.1 competence type IV pilus ATPase ComGA Machinery gene
  PYH69_RS07870 (PYH69_07870) - 1588803..1589051 (+) 249 WP_017000234.1 DUF2626 family protein -
  PYH69_RS07875 (PYH69_07875) - 1589116..1589748 (-) 633 WP_017000233.1 MBL fold metallo-hydrolase -
  PYH69_RS07880 (PYH69_07880) - 1589846..1590085 (+) 240 WP_282861669.1 DUF2759 domain-containing protein -
  PYH69_RS07885 (PYH69_07885) - 1590208..1591560 (-) 1353 WP_282861670.1 IS1182 family transposase -
  PYH69_RS07890 (PYH69_07890) - 1591768..1592082 (-) 315 WP_282861671.1 MTH1187 family thiamine-binding protein -
  PYH69_RS07895 (PYH69_07895) - 1592082..1593053 (-) 972 WP_017000230.1 ROK family glucokinase -
  PYH69_RS07900 (PYH69_07900) - 1593050..1593241 (-) 192 WP_017000229.1 YqgQ family protein -

Sequence

Protein


Download         Length: 298 a.a.        Molecular weight: 34435.59 Da        Isoelectric Point: 5.8696

>NTDB_id=795828 PYH69_RS07865 WP_017000235.1 1587754..1588650(-) (comGA) [Mammaliicoccus lentus strain 7048]
METLFKTIIKDAIILKATDIHFIPVKEDRVKVQLRISGENTIYDCDINNALYNRLLIYMKFIAHLDVSERNKAQSGQYIF
DDIYKYYLRISTLPLSLGIESCSIRLTPKYFEEYIDHNIDDNYKIIKEEMFNSEGLFLFTGPTGSGKSTIMYQFLYDLQQ
IKDKHIITIEDPVEQVLDGVVQVSVNEKANINYHNSFKAILRCDPDVVMIGEIRDEETAKQVIQASLSGHLILSTMHAKD
NLGAIYRLLELGISKEQIKQGIACIANQRLLNDQLGNRYRQMTYIFSHEINLYLENKS

Nucleotide


Download         Length: 897 bp        

>NTDB_id=795828 PYH69_RS07865 WP_017000235.1 1587754..1588650(-) (comGA) [Mammaliicoccus lentus strain 7048]
ATGGAAACTTTATTTAAGACAATTATCAAAGATGCTATTATTTTGAAAGCAACTGACATTCATTTTATTCCAGTGAAAGA
GGATCGGGTCAAAGTTCAGTTGAGGATAAGTGGTGAGAATACGATTTATGATTGTGATATTAATAACGCTTTATATAATC
GTTTATTAATTTATATGAAGTTTATTGCTCATCTAGATGTAAGTGAAAGGAATAAAGCTCAAAGTGGTCAATATATATTT
GATGATATATATAAATATTATTTAAGAATATCCACTTTACCTTTAAGTCTAGGAATTGAGTCTTGCTCAATTAGGTTAAC
GCCTAAATATTTCGAAGAATATATTGACCATAATATTGATGACAATTATAAAATAATTAAAGAAGAAATGTTTAATTCCG
AAGGCTTATTTTTGTTCACAGGACCAACCGGTTCGGGTAAATCGACGATTATGTACCAATTTTTATATGACTTACAACAA
ATTAAAGATAAACATATTATTACGATTGAAGACCCAGTTGAACAAGTTTTAGATGGTGTAGTACAAGTTTCTGTAAATGA
AAAAGCAAACATAAATTATCATAATTCTTTTAAAGCCATTTTAAGATGTGATCCAGATGTTGTCATGATAGGAGAAATTA
GAGATGAAGAAACAGCCAAGCAAGTTATACAAGCAAGTTTAAGTGGTCATTTAATATTATCTACAATGCATGCGAAAGAT
AATTTAGGGGCTATTTATAGACTTTTAGAACTTGGAATATCTAAAGAGCAAATAAAACAAGGTATCGCATGTATAGCCAA
TCAAAGACTTTTAAATGACCAATTAGGAAATAGATATAGACAAATGACTTATATATTTAGTCATGAAATTAATCTATATT
TAGAAAATAAATCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

50.993

100

0.517
  comGA Staphylococcus aureus N315

50.993

100

0.517
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

39.068

93.624

0.366