Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   PW252_RS02480 Genome accession   NZ_CP118735
Coordinates   477440..478732 (+) Length   430 a.a.
NCBI ID   WP_248049337.1    Uniprot ID   A0AA96VKN5
Organism   Streptococcus iners strain 29887     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 472440..483732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PW252_RS02450 (PW252_02450) - 474392..474607 (+) 216 WP_105144558.1 hypothetical protein -
  PW252_RS02455 (PW252_02455) - 474610..474960 (+) 351 WP_248049330.1 hypothetical protein -
  PW252_RS02460 (PW252_02460) - 474964..475389 (+) 426 WP_248049331.1 hypothetical protein -
  PW252_RS02465 (PW252_02465) - 475391..475630 (+) 240 WP_248049333.1 phage holin -
  PW252_RS02470 (PW252_02470) - 475736..476452 (+) 717 Protein_449 SH3 domain-containing protein -
  PW252_RS02475 (PW252_02475) - 476751..477383 (-) 633 WP_248049335.1 YigZ family protein -
  PW252_RS02480 (PW252_02480) comFA/cflA 477440..478732 (+) 1293 WP_248049337.1 DEAD/DEAH box helicase Machinery gene
  PW252_RS02485 (PW252_02485) comFC/cflB 478725..479390 (+) 666 WP_248049339.1 ComF family protein Machinery gene
  PW252_RS02490 (PW252_02490) hpf 479467..480009 (+) 543 WP_105117239.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48822.12 Da        Isoelectric Point: 8.3469

>NTDB_id=794729 PW252_RS02480 WP_248049337.1 477440..478732(+) (comFA/cflA) [Streptococcus iners strain 29887]
MKELENYYGRLFTKYQLTPAEREKAEKLSSVTDKHACFRCGTAFEEENKLPNDAYYCRACLLLGRVRSDEELYHFPQEEF
PVNRCLKWKGTLTDWQQMISDGLVENVGKRQATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQD
DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYRAADNAVKEDGVQVFLTATSTDEL
DRKVKTGKLKRLSLPRRFHGNPLVVPQKVWFSKFDANLKKQKLAPKLTRAIQEQRKTGHPLLIFVPEISKGQDFAKIMEK
TFPEETVGFVSSQTENRLDIVEGFRNQKITILISTTILERGVTFPKVDVIVVQANHYLYTSSSLVQIAGRVGRSIDRPTG
LLQFFHEGSTRSIEKAIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=794729 PW252_RS02480 WP_248049337.1 477440..478732(+) (comFA/cflA) [Streptococcus iners strain 29887]
ATGAAAGAATTAGAAAATTATTATGGAAGACTATTTACCAAATACCAATTGACCCCAGCAGAGCGAGAAAAAGCAGAGAA
GCTATCCAGTGTCACCGACAAGCATGCCTGCTTTCGCTGTGGTACAGCTTTTGAAGAAGAAAATAAATTGCCAAATGATG
CCTACTACTGTCGAGCCTGTTTGCTTCTAGGCAGAGTACGGTCAGATGAAGAACTCTATCATTTTCCACAGGAAGAATTT
CCTGTAAATAGGTGTTTGAAATGGAAGGGGACATTAACAGATTGGCAGCAAATGATTTCAGATGGATTGGTTGAGAATGT
AGGAAAAAGACAGGCTACACTGGTTCATGCTGTAACAGGAGCAGGAAAAACGGAAATGATTTATCATACAGTTGCATCCG
TGATTGATAAAGGAGGGGCAGTCTGTCTAGCTAGTCCTCGAATAGACGTCTGCATAGAACTCTATAAGCGCCTTCAAGAT
GACTTCTCAGTACCGATTAGTTTGCTACATGGAGAGTCAGAACCCTATTTCCGAACGCCGCTTGTTGTAGCGACCACCCA
TCAGTTACTAAAATTTTACCAGGCCTTTGATTTGGTTTTGATTGACGAAGTCGACGCCTTTCCCTATGCTGACAACCCCA
TGCTCTACCGTGCTGCTGATAACGCGGTTAAGGAAGACGGTGTCCAAGTATTTTTGACGGCAACGTCAACAGATGAACTG
GACAGAAAAGTCAAAACAGGCAAATTAAAACGGCTCAGCCTCCCCAGACGATTTCACGGCAATCCACTGGTTGTTCCGCA
AAAGGTTTGGTTTTCCAAATTTGATGCCAATCTAAAAAAACAGAAATTAGCCCCCAAACTTACTCGAGCCATTCAAGAAC
AAAGAAAAACAGGTCACCCCTTGCTCATATTTGTGCCAGAAATTTCCAAAGGTCAAGATTTTGCTAAGATAATGGAAAAA
ACATTTCCAGAAGAAACTGTTGGCTTTGTATCCAGTCAAACTGAAAATCGACTTGATATAGTAGAAGGCTTTCGCAATCA
AAAGATTACCATTCTCATTTCTACCACCATTTTAGAACGTGGTGTCACCTTTCCAAAAGTAGATGTTATAGTGGTCCAAG
CCAATCATTACCTCTACACATCGTCTAGTCTTGTACAGATTGCTGGTCGTGTAGGTCGAAGTATAGACCGTCCGACAGGC
TTGCTCCAATTTTTCCACGAGGGGTCTACTCGTTCCATTGAAAAGGCTATTGCAGAAATCAAACAAATGAACAAGGAGGC
AGGCTATGTCTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(91-235)

Helicase conserved C-terminal domain (Helicase_C)

(289-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

65.893

100

0.66
  comFA/cflA Streptococcus mitis SK321

65.893

100

0.66
  comFA/cflA Streptococcus pneumoniae Rx1

64.965

100

0.651
  comFA/cflA Streptococcus pneumoniae D39

64.965

100

0.651
  comFA/cflA Streptococcus pneumoniae R6

64.965

100

0.651
  comFA/cflA Streptococcus pneumoniae TIGR4

64.965

100

0.651
  comFA Lactococcus lactis subsp. cremoris KW2

52.696

94.884

0.5
  comFA Latilactobacillus sakei subsp. sakei 23K

38.695

99.767

0.386