Detailed information NTG0794049

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: PWF74_RS03075
• Genome accession: NZ_CP118627
• Coordinates: 611167..612423 (-)
• Length: 418 a.a.
• NCBI ID: WP_165705905.1
• Uniprot ID: A0AAX3NCX3
• Organism: Lactococcus garvieae strain Pa-2
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 606167..617423

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWF74_RS03040 (PWF74_03040)	rpmA	606288..606572 (+)	285	WP_004257956.1	50S ribosomal protein L27	-
PWF74_RS03045 (PWF74_03045)	ald	606922..608028 (+)	1107	WP_165705902.1	alanine dehydrogenase	-
PWF74_RS03050 (PWF74_03050)	-	608146..609105 (+)	960	WP_141920987.1	PhoH family protein	-
PWF74_RS03055 (PWF74_03055)	-	609107..609580 (+)	474	WP_014024618.1	NUDIX hydrolase	-
PWF74_RS03060 (PWF74_03060)	ybeY	609590..610078 (+)	489	WP_165705903.1	rRNA maturation RNase YbeY	-
PWF74_RS03065 (PWF74_03065)	-	610062..610523 (+)	462	WP_042218685.1	diacylglycerol kinase family protein	-
PWF74_RS03070 (PWF74_03070)	-	610526..611170 (-)	645	WP_165705904.1	ComF family protein	-
PWF74_RS03075 (PWF74_03075)	comFA	611167..612423 (-)	1257	WP_165705905.1	DEAD/DEAH box helicase	Machinery gene
PWF74_RS03080 (PWF74_03080)	-	612478..613101 (+)	624	WP_004257938.1	YigZ family protein	-
PWF74_RS03085 (PWF74_03085)	-	613115..614137 (+)	1023	WP_165705906.1	DUF475 domain-containing protein	-
PWF74_RS03090 (PWF74_03090)	-	614168..615190 (-)	1023	WP_165705907.1	alpha/beta hydrolase	-
PWF74_RS03095 (PWF74_03095)	-	615510..616289 (+)	780	WP_019292766.1	formate/nitrite transporter family protein	-
PWF74_RS03100 (PWF74_03100)	glyQ	616303..617271 (+)	969	WP_004257927.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 47801.46 Da
• Isoelectric Point: 9.8475

>NTDB_id=794049 PWF74_RS03075 WP_165705905.1 611167..612423(-) (comFA) [Lactococcus garvieae strain Pa-2]
MDELYGRLLLQKELTKLPDKATLFDGMQDVSKTVMMCNRCGKKIKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FSAASSLNWQGKLTASQQHVSDSLVKLHQQQKTVLVQAVTGAGKTEMIYQSIDNTLKEGKAVGLTSPRIDVCLELYHRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLLILDEVDAFPFPDNEMLYFALTQARKPSSSLIYLTATTTDN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKIVKKQRESGFPLLIFVPEIRQGEKLCQDLQSNFPHEEIAFVAS
TSPERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPEGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFL

Nucleotide

• Length: 1257 bp

>NTDB_id=794049 PWF74_RS03075 WP_165705905.1 611167..612423(-) (comFA) [Lactococcus garvieae strain Pa-2]
ATGGATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGACGAAACTTCCAGATAAAGCAACACTTTTTGATGGGAT
GCAAGATGTTTCCAAAACAGTGATGATGTGCAACCGCTGTGGCAAAAAGATCAAGAAAAAAGAGGTCCTTCTCCCTGTTG
GTGCTTATTATTGTCCTCATTGTATTCAAATGGGACGTGTGCGCTCGGATGAAAAACTTTACCACCTTCCACAGGAGGAT
TTTTCAGCAGCTTCTTCTCTCAACTGGCAGGGAAAACTCACAGCATCTCAGCAACATGTATCCGATAGCCTAGTCAAATT
GCATCAGCAACAAAAAACAGTACTGGTACAGGCTGTTACTGGTGCAGGAAAAACCGAAATGATTTATCAGAGCATTGATA
ACACCCTTAAAGAAGGAAAGGCTGTAGGTTTAACTAGCCCGCGTATTGATGTCTGTCTAGAGCTCTATCATCGCTTAAAG
AGAGATTTTTCTTGTCCCATCTCGCTTTTGCACGGAAAGAGTGAAAAATACAGCCGCTCGCCACTCGTAATTGCAACGAC
ACATCAGCTTATGCGTTTTCGCCATGCCTTTGACTTACTCATTCTTGATGAGGTGGATGCCTTTCCCTTTCCAGACAATG
AAATGCTTTATTTTGCACTGACTCAAGCTCGTAAACCTTCTTCAAGTTTGATTTATCTTACTGCAACCACCACCGATAAT
TTGGAAAAACAAGTCAAATTAGGACAAATCGAGAAGCTACAGCTTCCACGACGTTTTCATGGTTTTCCTCTGGTACTCCC
GCAATTTTTTTGGCAAAGCAAGTTTTACAAAATAGTCAAAAAACAACGCGAATCTGGTTTTCCCTTACTTATCTTTGTCC
CTGAAATAAGACAGGGAGAAAAACTCTGCCAAGATTTACAGAGTAATTTTCCTCATGAAGAAATTGCTTTTGTCGCGTCA
ACAAGTCCTGAACGCTTAGAGGCTGTGGAACGCTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACCATTTTAGA
ACGTGGTGTAACTTTCCCTAAGGTTGACGTTTTTGTGTTTCAAAGTCACCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGAGGGTAAGGTATTTTTCTTTCATCTAGGAAAAACAACAGCG
ATGTTGGAAGCCTATAAAAATATCAGAAATATGAATAAAGCAGGGGGGTTCCTATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (92-236)

• Helicase conserved C-terminal domain (Helicase_C) (274-382)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	57.653	93.78	0.541
comFA/cflA	Streptococcus mitis SK321	52.347	100	0.533
comFA/cflA	Streptococcus pneumoniae TIGR4	52.347	100	0.533
comFA/cflA	Streptococcus pneumoniae Rx1	52.347	100	0.533
comFA/cflA	Streptococcus pneumoniae D39	52.347	100	0.533
comFA/cflA	Streptococcus pneumoniae R6	52.347	100	0.533
comFA/cflA	Streptococcus mitis NCTC 12261	51.408	100	0.524
comFA	Bacillus subtilis subsp. subtilis str. 168	38.861	96.651	0.376
comFA	Latilactobacillus sakei subsp. sakei 23K	38.539	94.976	0.366