Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   PUN47_RS05065 Genome accession   NZ_CP118599
Coordinates   1096765..1099002 (+) Length   745 a.a.
NCBI ID   WP_274889203.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 10M-VF     
Function   ssDNA transport through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1091765..1104002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN47_RS05040 (PUN47_05040) - 1092216..1092785 (-) 570 WP_020430034.1 hypothetical protein -
  PUN47_RS05045 (PUN47_05045) lolC 1092961..1094169 (+) 1209 WP_032080004.1 lipoprotein-releasing ABC transporter permease subunit LolC -
  PUN47_RS05050 (PUN47_05050) lolD 1094162..1094848 (+) 687 WP_032080003.1 lipoprotein-releasing ABC transporter ATP-binding protein LolD -
  PUN47_RS05055 (PUN47_05055) lolE 1094849..1096093 (+) 1245 WP_202650102.1 lipoprotein-releasing ABC transporter permease subunit LolE -
  PUN47_RS05060 (PUN47_05060) - 1096226..1096756 (-) 531 WP_020327788.1 DUF2062 domain-containing protein -
  PUN47_RS05065 (PUN47_05065) comEC 1096765..1099002 (+) 2238 WP_274889203.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PUN47_RS05070 (PUN47_05070) msbA 1099035..1100783 (+) 1749 WP_020327786.1 lipid A ABC transporter ATP-binding protein/permease MsbA -
  PUN47_RS05075 (PUN47_05075) lpxK 1100787..1101794 (+) 1008 WP_202650105.1 tetraacyldisaccharide 4'-kinase -
  PUN47_RS05080 (PUN47_05080) - 1101775..1101954 (+) 180 WP_020327784.1 Trm112 family protein -
  PUN47_RS05085 (PUN47_05085) kdsB 1101954..1102697 (+) 744 WP_020327783.1 3-deoxy-manno-octulosonate cytidylyltransferase -

Sequence

Protein


Download         Length: 745 a.a.        Molecular weight: 83428.80 Da        Isoelectric Point: 8.6939

>NTDB_id=793846 PUN47_RS05065 WP_274889203.1 1096765..1099002(+) (comEC) [Vibrio fluvialis strain 10M-VF]
MTLYSNYWMLASFSLTVLSAPLWPWMPEWDFAFICLAALMTTLVVSRFRVFGGIALALLVIVTHGNVVRSQSNTIFQAGQ
DITIKGEVDSFFKQISYGYEGTVVVRSINGQELHTFWQPKVRLIAPVDLQIGDQFEFSVTVKPVYGRRNEAGFDLEAYYF
SQGWVARVNVKPQSKFEVVSTPNLRSMLHRQIKTWTQNSPSQGIILALTFGDRNGIHEAEWRSLRNSGLIHLVAISGLHI
GMAFAIGYLIGSAMMRLHVSLLWVPFVSGMCIAAIYAWLAGFTLPTQRALLMCALNVALTMSGMRVTAVQRILITLAAVL
IVTPFAPLSSSFWLSFLAVALVLYQLADRTTRHGWRKWLTMQCSLVTMMIPVSAYFFSGFSVSSALYNLIFIPYFSFVIV
PLLFLALFLTVFVGDITWLWRGIDLSFWPLRRALEWAGSSWIAVSQTATVVFSVGLLLWICRPILSWRVQLYAGIALLGL
GIFTPQDERWRVDILDVGHGLSVLIERNHNALLYDTGSSWPEGSYVRSLIVPILNQRGMDSLDGLILSHTDNDHAGGLKD
AETLLSPKWVVASQSAPNWQACHAGEQWEWQGLTMTALWPPQTVNRAYNLQSCVIRLSDPEYGHSLLLAGDVTAVGEWLL
SRQPMDIQSDVIIVPHHGSKTSSTRQFIERVSPQVAIASLAKGNQWQLPHRSVVARYLDAGSDWLDTGEAGQITLTYQAQ
SRQLSTLRQIGNPSWYRQMLRKGVE

Nucleotide


Download         Length: 2238 bp        

>NTDB_id=793846 PUN47_RS05065 WP_274889203.1 1096765..1099002(+) (comEC) [Vibrio fluvialis strain 10M-VF]
ATGACTCTCTATTCGAATTACTGGATGCTCGCTTCGTTCTCGCTCACCGTATTGTCTGCGCCCCTTTGGCCGTGGATGCC
AGAGTGGGATTTTGCATTCATTTGCCTTGCTGCTCTGATGACCACGCTCGTGGTGAGCCGATTCAGAGTTTTTGGCGGGA
TCGCGCTTGCCTTGTTAGTGATAGTAACACATGGCAACGTTGTGAGATCTCAATCCAATACTATTTTTCAAGCAGGGCAG
GATATTACCATAAAAGGTGAAGTTGACAGCTTTTTTAAGCAAATTAGTTATGGTTATGAAGGGACTGTAGTAGTCAGATC
AATCAATGGACAAGAATTGCACACTTTTTGGCAGCCGAAGGTGCGGTTAATTGCTCCGGTTGATCTGCAAATTGGCGATC
AGTTTGAGTTTTCTGTGACGGTGAAACCCGTTTATGGCCGCCGTAATGAGGCCGGTTTTGACTTAGAAGCTTACTACTTC
AGTCAGGGTTGGGTTGCGAGAGTGAACGTTAAACCACAGTCAAAATTTGAGGTCGTTTCAACGCCGAATTTGCGCAGTAT
GTTGCACCGTCAAATCAAAACATGGACGCAAAATAGCCCGTCACAAGGCATAATCTTGGCGCTGACATTTGGTGACCGTA
ACGGCATCCATGAAGCTGAATGGCGCTCACTGAGAAACAGCGGACTCATTCATCTGGTGGCGATATCCGGTTTGCATATT
GGCATGGCGTTCGCGATTGGTTATCTGATTGGAAGTGCGATGATGCGGCTGCATGTCAGTTTGCTGTGGGTGCCTTTCGT
TTCCGGGATGTGTATTGCCGCGATTTATGCCTGGCTGGCAGGATTTACCTTACCGACCCAGCGTGCTTTGTTGATGTGCG
CTCTCAACGTCGCTCTCACGATGAGTGGTATGCGTGTGACGGCGGTGCAACGAATCCTTATCACCCTTGCTGCGGTATTA
ATCGTGACGCCATTTGCGCCGCTCTCCAGCAGTTTCTGGCTCTCATTCTTGGCGGTCGCGTTGGTTTTGTATCAATTGGC
AGACCGAACAACGCGTCATGGCTGGCGAAAATGGCTGACGATGCAATGCTCGTTAGTGACCATGATGATTCCGGTTTCCG
CATATTTTTTCTCCGGGTTCAGTGTTTCGTCGGCGCTCTACAACCTGATTTTTATTCCTTACTTCAGCTTTGTCATTGTG
CCGCTGCTGTTTCTCGCACTGTTTCTCACTGTCTTTGTCGGAGATATCACCTGGCTGTGGCGTGGGATTGACCTGAGCTT
CTGGCCACTGCGGCGGGCACTGGAATGGGCGGGATCGAGCTGGATTGCTGTAAGTCAGACGGCGACGGTCGTATTTAGCG
TTGGCCTTCTCCTTTGGATTTGCCGCCCGATATTAAGCTGGCGAGTTCAACTCTATGCGGGAATTGCGTTACTTGGCTTG
GGCATTTTTACCCCACAGGATGAGCGTTGGCGAGTTGACATCCTTGATGTCGGGCACGGTCTGTCAGTGTTGATTGAACG
GAATCACAACGCCCTGTTGTACGACACTGGCAGTAGCTGGCCTGAAGGCAGTTACGTTCGCTCTTTGATAGTTCCAATAC
TCAATCAGCGCGGTATGGACTCTCTGGATGGTTTAATTCTCAGTCATACAGACAATGACCACGCTGGTGGTTTAAAAGAT
GCCGAAACCTTATTGTCCCCGAAATGGGTTGTGGCGAGTCAGTCTGCTCCAAATTGGCAGGCTTGCCACGCGGGTGAACA
GTGGGAATGGCAAGGACTGACGATGACAGCACTGTGGCCGCCGCAAACCGTCAATCGTGCTTATAATCTGCAGTCGTGCG
TGATTCGATTAAGCGATCCTGAATACGGGCATTCACTACTTCTGGCTGGCGATGTTACAGCAGTCGGTGAGTGGTTACTC
AGTCGGCAACCCATGGATATTCAGAGCGATGTCATCATTGTGCCGCATCACGGCAGTAAAACGTCCTCAACACGTCAGTT
TATCGAGCGGGTTTCCCCGCAAGTGGCTATTGCATCTCTGGCGAAAGGGAATCAATGGCAGTTGCCGCATCGCAGTGTGG
TGGCGCGTTATCTGGATGCGGGGTCCGACTGGCTTGATACTGGCGAGGCGGGGCAAATCACGCTGACCTATCAAGCTCAA
TCCCGGCAGCTATCGACTCTGCGCCAAATAGGAAATCCTTCATGGTATAGGCAGATGCTACGTAAAGGGGTAGAATGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(208-461)

Domain of unknown function (DUF4131) (DUF4131)

(32-173)

Metallo-beta-lactamase superfamily (Lactamase_B)

(497-678)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Vibrio cholerae strain A1552

53.918

100

0.545
  comEC Vibrio parahaemolyticus RIMD 2210633

42.364

100

0.428
  comEC Vibrio campbellii strain DS40M4

41.27

100

0.419