Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PWW25_RS12735 Genome accession   NZ_CP118584
Coordinates   2708505..2709521 (-) Length   338 a.a.
NCBI ID   WP_029789968.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2703505..2714521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW25_RS12710 aroK 2704361..2704879 (-) 519 WP_005451507.1 shikimate kinase AroK -
  PWW25_RS12715 pilQ 2705069..2706820 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ family protein Machinery gene
  PWW25_RS12720 pilP 2706855..2707370 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PWW25_RS12725 pilO 2707360..2707953 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PWW25_RS12730 pilN 2707946..2708521 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PWW25_RS12735 pilM 2708505..2709521 (-) 1017 WP_029789968.1 type IV pilus assembly protein PilM Machinery gene
  PWW25_RS12740 - 2709700..2712270 (+) 2571 WP_017819671.1 PBP1A family penicillin-binding protein -
  PWW25_RS12745 oxyR 2712337..2713245 (-) 909 WP_274876482.1 DNA-binding transcriptional regulator OxyR -
  PWW25_RS12750 - 2713420..2714148 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38023.40 Da        Isoelectric Point: 6.4206

>NTDB_id=793671 PWW25_RS12735 WP_029789968.1 2708505..2709521(-) (pilM) [Vibrio harveyi strain fish5]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDGYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=793671 PWW25_RS12735 WP_029789968.1 2708505..2709521(-) (pilM) [Vibrio harveyi strain fish5]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGACAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACG
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAGGTTGCTACCGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-326)

Type IV pilus assembly protein PilM; (PilM_2)

(8-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.533

100

0.905
  pilM Vibrio cholerae strain A1552

48.83

100

0.494