Detailed information NTG0793624

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PWW29_RS15870
• Genome accession: NZ_CP118582
• Coordinates: 3416144..3417013 (+)
• Length: 289 a.a.
• NCBI ID: WP_005446255.1
• Uniprot ID: A0A3A1Q6Q3
• Organism: Vibrio harveyi strain fish4
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3411144..3422013

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWW29_RS15850	nadC	3411510..3412397 (+)	888	WP_029790346.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PWW29_RS15855	-	3412662..3413129 (+)	468	WP_050933475.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
PWW29_RS15860	pilB	3413129..3414814 (+)	1686	WP_017189132.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PWW29_RS15865	pilC	3414848..3416071 (+)	1224	WP_274883216.1	type II secretion system F family protein	Machinery gene
PWW29_RS15870	pilD	3416144..3417013 (+)	870	WP_005446255.1	A24 family peptidase	Machinery gene
PWW29_RS15875	coaE	3417014..3417628 (+)	615	WP_009703756.1	dephospho-CoA kinase	-
PWW29_RS15880	zapD	3417656..3418396 (+)	741	WP_005446251.1	cell division protein ZapD	-
PWW29_RS15885	yacG	3418453..3418653 (+)	201	WP_026000212.1	DNA gyrase inhibitor YacG	-
PWW29_RS15890	rplS	3418942..3419295 (-)	354	WP_005379971.1	50S ribosomal protein L19	-
PWW29_RS15895	trmD	3419337..3420080 (-)	744	WP_005446247.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
PWW29_RS15900	rimM	3420108..3420656 (-)	549	WP_005446245.1	ribosome maturation factor RimM	-
PWW29_RS15905	rpsP	3420685..3420933 (-)	249	WP_004410028.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32536.74 Da
• Isoelectric Point: 7.5422

>NTDB_id=793624 PWW29_RS15870 WP_005446255.1 3416144..3417013(+) (pilD) [Vibrio harveyi strain fish4]
MEVFQYYPWLFVVFTAIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIEPPKETLTLSVPRSSCQQCGTHIRIR
DNIPVISWLLLKGKCHNCQSPISARYPLIELLTAFCSGFIAFHFGFSYFTVALVFFTFVLIAATFIDLDTMLLPDQLTLP
LMWAGIALALAGISPVSLQDSIIGAMAGYLCLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLIQLRLQKQGIEKAFPFGPYLAIAGWVSLIWGNQILNWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=793624 PWW29_RS15870 WP_005446255.1 3416144..3417013(+) (pilD) [Vibrio harveyi strain fish4]
ATGGAAGTATTCCAGTACTACCCATGGCTATTCGTTGTATTTACCGCTATTTTTGGTTTGATCGTGGGTAGTTTCCTCAA
CGTAGTTATTTACCGTTTACCTAAGATCATGGAACTAGAATGGCGACGCGAGTGCGCTGAATCATTCCCTGAATACAAGA
TCGAGCCACCAAAAGAGACATTAACCTTAAGCGTGCCACGTTCATCTTGTCAGCAATGCGGTACACATATCCGCATTAGA
GACAATATCCCTGTGATTAGTTGGCTATTACTAAAAGGTAAATGCCACAACTGCCAATCACCTATCAGTGCCCGTTACCC
TCTTATCGAACTGCTTACCGCTTTTTGTTCGGGTTTTATCGCTTTTCATTTCGGTTTTAGCTACTTCACTGTGGCGCTGG
TTTTCTTTACCTTTGTATTGATTGCTGCCACATTTATCGACCTTGATACCATGTTGCTGCCAGACCAACTCACACTGCCA
TTGATGTGGGCGGGTATTGCTCTTGCTCTTGCCGGTATCAGCCCTGTGAGTCTTCAAGATTCTATTATTGGAGCAATGGC
AGGCTACTTATGTCTTTGGAGTGTTTATTGGTTATTCAAACTCCTCACAGGCAAAGAGGGAATGGGCTATGGTGACTTTA
AACTCCTTGCTGCATTAGGAGCTTGGCTGGGCTGGCAATCTCTGCCGATGATCATTTTACTTTCTTCGGTAGTAGGCGTC
ATTTTTGGTCTTATCCAGCTGCGCTTGCAAAAGCAAGGTATCGAAAAAGCCTTTCCTTTTGGTCCTTACCTTGCAATTGC
AGGATGGGTGAGCCTAATTTGGGGCAATCAAATCCTCAACTGGTACTTTACGTCGATTCTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A3A1Q6Q3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	97.232	100	0.972
pilD	Vibrio cholerae strain A1552	74.306	99.654	0.74
pilD	Acinetobacter baumannii D1279779	52.768	93.772	0.495
pilD	Acinetobacter nosocomialis M2	52.399	93.772	0.491
pilD	Neisseria gonorrhoeae MS11	45.652	95.502	0.436