Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PWW32_RS00765 Genome accession   NZ_CP118580
Coordinates   157665..158258 (-) Length   197 a.a.
NCBI ID   WP_274792385.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 152665..163258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW32_RS00745 aroB 153534..154634 (-) 1101 WP_274792386.1 3-dehydroquinate synthase -
  PWW32_RS00750 aroK 154666..155184 (-) 519 WP_005451507.1 shikimate kinase AroK -
  PWW32_RS00755 pilQ 155374..157125 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ family protein Machinery gene
  PWW32_RS00760 pilP 157160..157675 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PWW32_RS00765 pilO 157665..158258 (-) 594 WP_274792385.1 type 4a pilus biogenesis protein PilO Machinery gene
  PWW32_RS00770 pilN 158251..158826 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PWW32_RS00775 pilM 158810..159826 (-) 1017 WP_029789968.1 type IV pilus assembly protein PilM Machinery gene
  PWW32_RS00780 - 160005..162575 (+) 2571 WP_274792384.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23021.56 Da        Isoelectric Point: 4.7081

>NTDB_id=793554 PWW32_RS00765 WP_274792385.1 157665..158258(-) (pilO) [Vibrio harveyi strain fish3]
MVDWQDLEMDEIAEWPLVPQIVVILLLVVLIQGLGYWFYLKPEQEQLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKQSEHFLYRLPLDIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVKANTYQFKPEVKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=793554 PWW32_RS00765 WP_274792385.1 157665..158258(-) (pilO) [Vibrio harveyi strain fish3]
ATGGTTGATTGGCAAGATCTAGAAATGGATGAGATCGCTGAGTGGCCATTGGTGCCTCAGATAGTAGTGATCTTGTTGTT
AGTTGTGCTGATTCAAGGCCTTGGTTATTGGTTTTATTTAAAGCCAGAGCAAGAACAGCTGAATAACCTTATTCAAGAAG
AACAAACCTTAAAAGTTGCGTTACGGATCAAAGCGAATAAAGTCGCTGCTTTGCCTCAATTGCAATCGCAACTTGATGAG
CTTACTACTCGATACGAATTGCTACTGCAACAACTGCCTGCACAGAAAGAGCTGGCAACCATGTTGGCTTCGGTGAATGA
ACTCGGAATTGATAATAAGTTGACGTTCACCCGAATCGACTGGGGTGATAAGCAATCAGAACACTTCCTTTATCGTTTAC
CGCTCGACATTGAGTTGACAGGGGATTTTCATGATATCGGTCGTTTCTCCCAAGCGATTGCGACGTTGCCACGCATCATC
ACTTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTCTGCACTTTCGTGTGAAAGCAAACACTTACCA
GTTCAAACCAGAGGTGAAGAAAGATGAGAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

92.632

96.447

0.893
  pilO Vibrio cholerae strain A1552

71.066

100

0.711