Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PWW32_RS00775 Genome accession   NZ_CP118580
Coordinates   158810..159826 (-) Length   338 a.a.
NCBI ID   WP_029789968.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 153810..164826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW32_RS00750 aroK 154666..155184 (-) 519 WP_005451507.1 shikimate kinase AroK -
  PWW32_RS00755 pilQ 155374..157125 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ family protein Machinery gene
  PWW32_RS00760 pilP 157160..157675 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PWW32_RS00765 pilO 157665..158258 (-) 594 WP_274792385.1 type 4a pilus biogenesis protein PilO Machinery gene
  PWW32_RS00770 pilN 158251..158826 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PWW32_RS00775 pilM 158810..159826 (-) 1017 WP_029789968.1 type IV pilus assembly protein PilM Machinery gene
  PWW32_RS00780 - 160005..162575 (+) 2571 WP_274792384.1 PBP1A family penicillin-binding protein -
  PWW32_RS00785 oxyR 162642..163550 (-) 909 WP_274792383.1 DNA-binding transcriptional regulator OxyR -
  PWW32_RS00790 - 163725..164453 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38023.40 Da        Isoelectric Point: 6.4206

>NTDB_id=793556 PWW32_RS00775 WP_029789968.1 158810..159826(-) (pilM) [Vibrio harveyi strain fish3]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDGYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=793556 PWW32_RS00775 WP_029789968.1 158810..159826(-) (pilM) [Vibrio harveyi strain fish3]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGACAACGCT
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACG
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAACAAGACCCTCTTGCTGAAGACACGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-326)

Type IV pilus assembly protein PilM; (PilM_2)

(8-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.533

100

0.905
  pilM Vibrio cholerae strain A1552

48.83

100

0.494