Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PWS04_RS07840 Genome accession   NZ_CP118528
Coordinates   1634123..1634716 (+) Length   197 a.a.
NCBI ID   WP_274792385.1    Uniprot ID   -
Organism   Vibrio harveyi strain 94/17     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1629123..1639716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWS04_RS07825 - 1629806..1632376 (-) 2571 WP_274792384.1 PBP1A family penicillin-binding protein -
  PWS04_RS07830 pilM 1632555..1633571 (+) 1017 WP_029789968.1 type IV pilus assembly protein PilM Machinery gene
  PWS04_RS07835 pilN 1633555..1634130 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PWS04_RS07840 pilO 1634123..1634716 (+) 594 WP_274792385.1 type 4a pilus biogenesis protein PilO Machinery gene
  PWS04_RS07845 pilP 1634706..1635221 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PWS04_RS07850 pilQ 1635256..1637007 (+) 1752 WP_005451505.1 type IV pilus secretin PilQ family protein Machinery gene
  PWS04_RS07855 aroK 1637197..1637715 (+) 519 WP_005451507.1 shikimate kinase AroK -
  PWS04_RS07860 aroB 1637747..1638847 (+) 1101 WP_274792386.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23021.56 Da        Isoelectric Point: 4.7081

>NTDB_id=792883 PWS04_RS07840 WP_274792385.1 1634123..1634716(+) (pilO) [Vibrio harveyi strain 94/17]
MVDWQDLEMDEIAEWPLVPQIVVILLLVVLIQGLGYWFYLKPEQEQLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKQSEHFLYRLPLDIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVKANTYQFKPEVKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=792883 PWS04_RS07840 WP_274792385.1 1634123..1634716(+) (pilO) [Vibrio harveyi strain 94/17]
ATGGTTGATTGGCAAGATCTAGAAATGGATGAGATCGCTGAGTGGCCATTGGTGCCTCAGATAGTAGTGATCTTGTTGTT
AGTTGTGCTGATTCAAGGCCTTGGTTATTGGTTTTATTTAAAGCCAGAGCAAGAACAGCTGAATAACCTTATTCAAGAAG
AACAAACCTTAAAAGTTGCGTTACGGATCAAAGCGAATAAAGTCGCTGCTTTGCCTCAATTGCAATCGCAACTTGATGAG
CTTACTACTCGATACGAATTGCTACTGCAACAACTGCCTGCACAGAAAGAGCTGGCAACCATGTTGGCTTCGGTGAATGA
ACTCGGAATTGATAATAAGTTGACGTTCACCCGAATCGACTGGGGTGATAAGCAATCAGAACACTTCCTTTATCGTTTAC
CGCTCGACATTGAGTTGACAGGGGATTTTCATGATATCGGTCGTTTCTCCCAAGCGATTGCGACGTTGCCACGCATCATC
ACTTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTCTGCACTTTCGTGTGAAAGCAAACACTTACCA
GTTCAAACCAGAGGTGAAGAAAGATGAGAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

92.632

96.447

0.893
  pilO Vibrio cholerae strain A1552

71.066

100

0.711