Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PVT72_RS11585 Genome accession   NZ_CP118495
Coordinates   2467465..2468535 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain FC02     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2462465..2473535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT72_RS11535 (PVT72_11535) - 2462524..2463108 (-) 585 WP_012117977.1 signal peptidase I -
  PVT72_RS11540 (PVT72_11540) tapA 2463080..2463751 (-) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  PVT72_RS11545 (PVT72_11545) - 2464010..2464339 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  PVT72_RS11550 (PVT72_11550) - 2464379..2464558 (-) 180 WP_003153093.1 YqzE family protein -
  PVT72_RS11555 (PVT72_11555) comGG 2464615..2464993 (-) 379 Protein_2229 competence type IV pilus minor pilin ComGG -
  PVT72_RS11560 (PVT72_11560) comGF 2464994..2465494 (-) 501 WP_274799405.1 competence type IV pilus minor pilin ComGF -
  PVT72_RS11565 (PVT72_11565) comGE 2465403..2465717 (-) 315 WP_274799406.1 competence type IV pilus minor pilin ComGE Machinery gene
  PVT72_RS11570 (PVT72_11570) comGD 2465701..2466138 (-) 438 WP_043867285.1 competence type IV pilus minor pilin ComGD Machinery gene
  PVT72_RS11575 (PVT72_11575) comGC 2466128..2466436 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  PVT72_RS11580 (PVT72_11580) comGB 2466441..2467478 (-) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  PVT72_RS11585 (PVT72_11585) comGA 2467465..2468535 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  PVT72_RS11590 (PVT72_11590) - 2468727..2469677 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  PVT72_RS11595 (PVT72_11595) - 2469823..2471124 (+) 1302 WP_264254869.1 hemolysin family protein -
  PVT72_RS11600 (PVT72_11600) - 2471163..2471993 (-) 831 WP_003153080.1 STAS domain-containing protein -
  PVT72_RS11610 (PVT72_11610) - 2472199..2472579 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PVT72_RS11615 (PVT72_11615) - 2472816..2473061 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=792797 PVT72_RS11585 WP_003153083.1 2467465..2468535(-) (comGA) [Bacillus velezensis strain FC02]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=792797 PVT72_RS11585 WP_003153083.1 2467465..2468535(-) (comGA) [Bacillus velezensis strain FC02]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGTTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGTTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTAAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCTATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGTGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374