Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   PWK65_RS00685 Genome accession   NZ_CP118481
Coordinates   104716..105150 (+) Length   144 a.a.
NCBI ID   WP_002987783.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 20185322     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99716..110150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWK65_RS00655 (PWK65_00645) - 101385..101750 (+) 366 WP_002986560.1 DUF1033 family protein -
  PWK65_RS00660 (PWK65_00650) comYA 101843..102781 (+) 939 WP_002987773.1 competence type IV pilus ATPase ComGA Machinery gene
  PWK65_RS00665 (PWK65_00655) comYB 102717..103751 (+) 1035 WP_223845212.1 competence type IV pilus assembly protein ComGB Machinery gene
  PWK65_RS00670 (PWK65_00660) comYC 103753..104079 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  PWK65_RS00675 (PWK65_00665) comGD 104054..104482 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  PWK65_RS00680 (PWK65_00670) comGE 104439..104723 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  PWK65_RS00685 (PWK65_00675) comYF 104716..105150 (+) 435 WP_002987783.1 competence type IV pilus minor pilin ComGF Machinery gene
  PWK65_RS00690 (PWK65_00680) comGG 105134..105460 (+) 327 WP_002987787.1 competence type IV pilus minor pilin ComGG -
  PWK65_RS00695 (PWK65_00685) comYH 105558..106511 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  PWK65_RS00700 (PWK65_00690) - 106570..107766 (+) 1197 WP_002987791.1 acetate kinase -
  PWK65_RS00705 (PWK65_00695) - 107953..108261 (+) 309 WP_002987795.1 hypothetical protein -
  PWK65_RS00710 (PWK65_00700) proC 108344..109114 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16585.23 Da        Isoelectric Point: 10.2064

>NTDB_id=792646 PWK65_RS00685 WP_002987783.1 104716..105150(+) (comYF) [Streptococcus pyogenes strain 20185322]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFCKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=792646 PWK65_RS00685 WP_002987783.1 104716..105150(+) (comYF) [Streptococcus pyogenes strain 20185322]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTCCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTACCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTGCAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGATATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396