Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PUW21_RS13860 Genome accession   NZ_CP118167
Coordinates   2652193..2652810 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain 21855     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2647193..2657810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW21_RS13830 (PUW21_13830) rpsT 2647494..2647760 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  PUW21_RS13835 (PUW21_13835) holA 2647775..2648818 (-) 1044 WP_015714286.1 DNA polymerase III subunit delta -
  PUW21_RS13840 (PUW21_13840) - 2648858..2649007 (-) 150 WP_003229985.1 hypothetical protein -
  PUW21_RS13845 (PUW21_13845) yqzM 2649048..2649182 (+) 135 WP_003229983.1 YqzM family protein -
  PUW21_RS13850 (PUW21_13850) comEC 2649223..2651553 (-) 2331 WP_069704087.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PUW21_RS13855 (PUW21_13855) comEB 2651557..2652126 (-) 570 WP_003229978.1 ComE operon protein 2 -
  PUW21_RS13860 (PUW21_13860) comEA 2652193..2652810 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  PUW21_RS13865 (PUW21_13865) comER 2652894..2653715 (+) 822 WP_014480313.1 late competence protein ComER -
  PUW21_RS13870 (PUW21_13870) yqeM 2653781..2654524 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  PUW21_RS13875 (PUW21_13875) rsfS 2654521..2654877 (-) 357 WP_014480315.1 ribosome silencing factor -
  PUW21_RS13880 (PUW21_13880) yqeK 2654895..2655455 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PUW21_RS13885 (PUW21_13885) nadD 2655445..2656014 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  PUW21_RS13890 (PUW21_13890) yhbY 2656026..2656316 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  PUW21_RS13895 (PUW21_13895) aroE 2656310..2657152 (-) 843 WP_041351695.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=790986 PUW21_RS13860 WP_004398514.1 2652193..2652810(-) (comEA) [Bacillus subtilis strain 21855]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=790986 PUW21_RS13860 WP_004398514.1 2652193..2652810(-) (comEA) [Bacillus subtilis strain 21855]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39