Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PUP75_RS26895 Genome accession   NZ_CP118145
Coordinates   5979112..5979984 (+) Length   290 a.a.
NCBI ID   WP_123412472.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain ATCC 17417     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5974112..5984984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP75_RS26880 (PUP75_26880) - 5976470..5976826 (-) 357 WP_009045666.1 BON domain-containing protein -
  PUP75_RS26885 (PUP75_26885) - 5977112..5977528 (-) 417 WP_007924673.1 pilin -
  PUP75_RS26890 (PUP75_26890) pilC 5977892..5979109 (+) 1218 WP_274335232.1 type II secretion system F family protein Machinery gene
  PUP75_RS26895 (PUP75_26895) pilD 5979112..5979984 (+) 873 WP_123412472.1 prepilin peptidase Machinery gene
  PUP75_RS26900 (PUP75_26900) coaE 5979981..5980604 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  PUP75_RS26905 (PUP75_26905) yacG 5980601..5980804 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  PUP75_RS26910 (PUP75_26910) - 5980809..5981024 (-) 216 WP_009045671.1 hypothetical protein -
  PUP75_RS26915 (PUP75_26915) - 5981090..5981779 (-) 690 WP_009045672.1 energy-coupling factor ABC transporter permease -
  PUP75_RS26920 (PUP75_26920) - 5981776..5982243 (-) 468 WP_053262707.1 hypothetical protein -
  PUP75_RS26925 (PUP75_26925) - 5982698..5984134 (+) 1437 WP_085534668.1 undecaprenyl-phosphate glucose phosphotransferase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32124.21 Da        Isoelectric Point: 7.4003

>NTDB_id=790557 PUP75_RS26895 WP_123412472.1 5979112..5979984(+) (pilD) [Pseudomonas chlororaphis strain ATCC 17417]
MSLTEVLASYPLVFVASVLVLGLIVGSFLNVLIWRLPKMLERDWHAQARDILGLPDEPVGPTYNLLLPHSQCPHCAHSIR
PWENIPLLSYLLLRGRCSVCQAPIGRRYPLVELTCGLASAFVAWHFGFGWQAGLMLFLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIVNGFELFVSLHDALWGAVAGYLCLWSVYWLFKLLTGKEGMGHGDFKLLAMFGAWGGWQILPMTLLLASLTG
ALAGVAMLRRNRAQMSTPIPFGPFLAIAGWIALLWGGQITDSYLQFVGFR

Nucleotide


Download         Length: 873 bp        

>NTDB_id=790557 PUP75_RS26895 WP_123412472.1 5979112..5979984(+) (pilD) [Pseudomonas chlororaphis strain ATCC 17417]
ATGTCATTGACTGAGGTCCTGGCCAGCTACCCGCTGGTCTTCGTAGCATCGGTGCTGGTCCTGGGCCTGATTGTCGGCAG
CTTTCTCAATGTGCTGATCTGGCGGCTGCCAAAGATGCTCGAGCGCGACTGGCACGCCCAGGCTCGCGACATCCTCGGGC
TGCCCGACGAGCCTGTCGGGCCGACCTACAACCTGTTGCTCCCGCACTCCCAGTGCCCGCATTGCGCGCACTCGATCCGC
CCCTGGGAAAACATCCCGCTGTTGAGCTACCTGCTGCTGCGCGGACGCTGCTCCGTATGCCAGGCCCCGATCGGCCGGCG
CTACCCGCTGGTGGAACTGACCTGCGGCCTGGCCTCGGCATTCGTGGCCTGGCATTTCGGCTTCGGCTGGCAGGCCGGCT
TGATGCTGTTCCTGAGCTGGGGCCTGTTGGCGATGAGCCTGATCGACGCCGATCATCAACTGTTGCCCGACGTTCTGGTG
CTGCCCCTGTTGTGGCTGGGCCTGATCGTCAATGGCTTCGAGCTGTTCGTCAGCCTCCACGATGCGCTGTGGGGCGCCGT
GGCCGGCTACCTGTGCCTGTGGTCGGTGTACTGGTTGTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGGCACGGCGATT
TCAAGCTGTTGGCCATGTTCGGCGCCTGGGGCGGCTGGCAGATTCTGCCGATGACCCTGCTGCTGGCCTCACTGACGGGA
GCCCTGGCCGGAGTCGCCATGCTGCGCCGCAACCGCGCGCAAATGTCCACGCCTATCCCTTTCGGTCCCTTTCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGACTCCTATTTGCAGTTTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.497

98.621

0.528
  pilD Vibrio campbellii strain DS40M4

51.93

98.276

0.51
  pilD Acinetobacter baumannii D1279779

49.815

93.448

0.466
  pilD Acinetobacter nosocomialis M2

49.815

93.448

0.466
  pilD Neisseria gonorrhoeae MS11

47.794

93.793

0.448