Detailed information NTG0790376

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PUP77_RS15860
• Genome accession: NZ_CP118138
• Coordinates: 3413313..3414185 (-)
• Length: 290 a.a.
• NCBI ID: WP_025807493.1
• Uniprot ID: -
• Organism: Pseudomonas chlororaphis strain DSM 295782
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3408313..3419185

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUP77_RS15830 (PUP77_15830)	-	3409162..3410598 (-)	1437	WP_053280964.1	undecaprenyl-phosphate glucose phosphotransferase	-
PUP77_RS15835 (PUP77_15835)	-	3411054..3411521 (+)	468	WP_274321580.1	FAD/FMN-containing dehydrogenase	-
PUP77_RS15840 (PUP77_15840)	-	3411518..3412207 (+)	690	WP_025807497.1	energy-coupling factor ABC transporter permease	-
PUP77_RS15845 (PUP77_15845)	-	3412273..3412488 (+)	216	WP_053280962.1	hypothetical protein	-
PUP77_RS15850 (PUP77_15850)	yacG	3412493..3412696 (-)	204	WP_025807495.1	DNA gyrase inhibitor YacG	-
PUP77_RS15855 (PUP77_15855)	coaE	3412693..3413316 (-)	624	WP_274321581.1	dephospho-CoA kinase	-
PUP77_RS15860 (PUP77_15860)	pilD	3413313..3414185 (-)	873	WP_025807493.1	prepilin peptidase	Machinery gene
PUP77_RS15865 (PUP77_15865)	pilC	3414188..3415405 (-)	1218	WP_274321582.1	type II secretion system F family protein	Machinery gene
PUP77_RS15870 (PUP77_15870)	-	3415771..3416187 (+)	417	WP_274321583.1	pilin	-
PUP77_RS15875 (PUP77_15875)	-	3416477..3416833 (+)	357	WP_053280958.1	BON domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32014.10 Da
• Isoelectric Point: 8.2548

>NTDB_id=790376 PUP77_RS15860 WP_025807493.1 3413313..3414185(-) (pilD) [Pseudomonas chlororaphis strain DSM 295782]
MSLTEVLASYPLVFVASVLVLGLIVGSFLNVLIWRLPKMLERDWHAQARDILGLPVEPAGPTYNLLLPHSQCPHCAHPIR
PWENIPVFSYLLLRGRCSACKTPIGRRYPLVELTCGLASAFVAWHFGFGWQAGLMLFLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIVNGFDLFVSLHDALWGAVAGYLCLWSVYWSFKLLTGKEGMGHGDFKLLAMFGAWGGWQILPMTLLLASLTG
ALAGVAMLRRNRAQMSTPIPFGPFLAIAGWIALLWGGQITGSYLQFVGFR

Nucleotide

• Length: 873 bp

>NTDB_id=790376 PUP77_RS15860 WP_025807493.1 3413313..3414185(-) (pilD) [Pseudomonas chlororaphis strain DSM 295782]
ATGTCATTGACTGAGGTCCTGGCCAGCTACCCGCTGGTCTTCGTAGCATCGGTGCTGGTCCTGGGCCTGATTGTCGGCAG
CTTCCTCAACGTGCTGATCTGGCGGCTGCCAAAGATGCTCGAGCGCGACTGGCATGCCCAGGCCCGGGACATCCTGGGGC
TGCCCGTGGAACCTGCCGGGCCGACCTACAACCTGTTGCTCCCACACTCCCAGTGCCCTCACTGCGCGCACCCGATCCGC
CCCTGGGAAAACATCCCAGTGTTCAGCTACCTGCTGCTGCGCGGACGCTGCTCGGCGTGCAAGACCCCGATCGGCCGGCG
CTACCCGCTGGTGGAGCTGACCTGCGGCCTGGCCTCGGCGTTCGTGGCCTGGCATTTCGGCTTTGGCTGGCAGGCCGGCT
TGATGCTGTTCCTGAGCTGGGGCCTGTTGGCGATGAGCCTGATCGATGCCGATCATCAACTGTTGCCCGACGTGCTGGTG
CTGCCCCTGTTGTGGCTGGGCCTGATCGTCAATGGTTTCGACCTGTTCGTCAGCCTCCACGATGCGCTGTGGGGCGCCGT
GGCCGGCTACCTGTGCCTGTGGTCGGTGTACTGGTCGTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGGCACGGCGATT
TCAAGCTGTTGGCCATGTTCGGCGCCTGGGGCGGCTGGCAGATTCTGCCGATGACCCTGCTGCTGGCCTCACTGACGGGA
GCTCTAGCCGGGGTTGCCATGCTGCGCCGCAACCGCGCGCAAATGTCCACGCCTATCCCTTTTGGTCCCTTTCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGGCTCCTATTTGCAGTTTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.004	97.586	0.517
pilD	Vibrio campbellii strain DS40M4	51.064	97.241	0.497
pilD	Acinetobacter baumannii D1279779	49.815	93.448	0.466
pilD	Acinetobacter nosocomialis M2	49.077	93.448	0.459
pilD	Neisseria gonorrhoeae MS11	48.162	93.793	0.452