Detailed information NTG0790298

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PUP76_RS15010
• Genome accession: NZ_CP118135
• Coordinates: 3259714..3260586 (-)
• Length: 290 a.a.
• NCBI ID: WP_274302094.1
• Uniprot ID: -
• Organism: Pseudomonas chlororaphis strain NCCB 820532
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3254714..3265586

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUP76_RS14980 (PUP76_14980)	-	3255564..3257000 (-)	1437	WP_274302092.1	undecaprenyl-phosphate glucose phosphotransferase	-
PUP76_RS14985 (PUP76_14985)	-	3257455..3257922 (+)	468	WP_053262707.1	hypothetical protein	-
PUP76_RS14990 (PUP76_14990)	-	3257919..3258608 (+)	690	WP_274302093.1	energy-coupling factor ABC transporter permease	-
PUP76_RS14995 (PUP76_14995)	-	3258674..3258889 (+)	216	WP_053262705.1	hypothetical protein	-
PUP76_RS15000 (PUP76_15000)	yacG	3258894..3259097 (-)	204	WP_009045670.1	DNA gyrase inhibitor YacG	-
PUP76_RS15005 (PUP76_15005)	coaE	3259094..3259717 (-)	624	WP_009045669.1	dephospho-CoA kinase	-
PUP76_RS15010 (PUP76_15010)	pilD	3259714..3260586 (-)	873	WP_274302094.1	prepilin peptidase	Machinery gene
PUP76_RS15015 (PUP76_15015)	pilC	3260589..3261806 (-)	1218	WP_274302095.1	type II secretion system F family protein	Machinery gene
PUP76_RS15020 (PUP76_15020)	-	3262170..3262586 (+)	417	WP_007924673.1	pilin	-
PUP76_RS15025 (PUP76_15025)	-	3262872..3263228 (+)	357	WP_009045666.1	BON domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32122.28 Da
• Isoelectric Point: 7.7036

>NTDB_id=790298 PUP76_RS15010 WP_274302094.1 3259714..3260586(-) (pilD) [Pseudomonas chlororaphis strain NCCB 820532]
MSLTEVLASYPLVFVASVLVLGLIVGSFLNVLIWRLPKMLERDWHAQARDILGLPDEPVGPTYNLLLPHSQCPHCAHPIR
LWENIPLLSYLLLRGRCSACKAPIGRRYPLVELTCGLASAFVAWHFGFGWQAGLMLFLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIVNGFELFVSLHDALWGAVAGYLCLWSVYWLFKLLTGKEGMGHGDFKLLAMFGAWGGWQILPMTLLLASLTG
ALAGVAMLRRNRAQMSTPIPFGPFLAIAGWIALLWGGQITDSYLQFVGFR

Nucleotide

• Length: 873 bp

>NTDB_id=790298 PUP76_RS15010 WP_274302094.1 3259714..3260586(-) (pilD) [Pseudomonas chlororaphis strain NCCB 820532]
ATGTCATTGACTGAGGTCCTGGCCAGCTACCCGCTGGTCTTCGTAGCATCGGTGCTGGTCCTGGGCCTGATTGTCGGCAG
CTTTCTCAATGTGCTGATCTGGCGGCTGCCAAAGATGCTCGAGCGCGACTGGCACGCCCAGGCCCGTGACATCCTCGGGC
TGCCCGACGAGCCTGTCGGGCCGACCTATAACCTGTTGCTCCCACACTCCCAGTGCCCGCACTGTGCGCACCCGATCCGC
CTCTGGGAAAACATCCCGCTGTTGAGCTACCTGCTGCTGCGCGGACGCTGCTCCGCATGCAAGGCCCCGATCGGCCGGCG
CTACCCGCTGGTGGAGCTGACCTGCGGCCTGGCCTCGGCATTCGTGGCCTGGCATTTCGGCTTCGGCTGGCAGGCCGGCT
TGATGCTGTTCCTGAGCTGGGGCCTGTTGGCGATGAGCCTGATCGATGCCGATCATCAACTGTTGCCCGACGTGCTGGTG
CTGCCCCTGTTGTGGCTGGGCCTGATCGTCAATGGCTTCGAGCTGTTCGTCAGCCTCCACGATGCGCTGTGGGGCGCCGT
GGCCGGCTACCTGTGCCTGTGGTCGGTGTACTGGTTGTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGGCACGGCGATT
TCAAGCTGTTGGCCATGTTCGGCGCCTGGGGCGGCTGGCAGATTCTGCCGATGACCCTGCTGCTGGCCTCACTGACGGGA
GCCCTGGCCGGAGTCGCCATGCTGCGCCGCAACCGCGCGCAAATGTCCACGCCTATCCCTTTCGGTCCCTTTCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGACTCCTATTTGCAGTTTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.196	98.621	0.534
pilD	Vibrio campbellii strain DS40M4	51.579	98.276	0.507
pilD	Acinetobacter baumannii D1279779	49.815	93.448	0.466
pilD	Acinetobacter nosocomialis M2	49.815	93.448	0.466
pilD	Neisseria gonorrhoeae MS11	47.794	93.793	0.448