Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PUP76_RS15015 Genome accession   NZ_CP118135
Coordinates   3260589..3261806 (-) Length   405 a.a.
NCBI ID   WP_274302095.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain NCCB 820532     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3255589..3266806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP76_RS14985 (PUP76_14985) - 3257455..3257922 (+) 468 WP_053262707.1 hypothetical protein -
  PUP76_RS14990 (PUP76_14990) - 3257919..3258608 (+) 690 WP_274302093.1 energy-coupling factor ABC transporter permease -
  PUP76_RS14995 (PUP76_14995) - 3258674..3258889 (+) 216 WP_053262705.1 hypothetical protein -
  PUP76_RS15000 (PUP76_15000) yacG 3258894..3259097 (-) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  PUP76_RS15005 (PUP76_15005) coaE 3259094..3259717 (-) 624 WP_009045669.1 dephospho-CoA kinase -
  PUP76_RS15010 (PUP76_15010) pilD 3259714..3260586 (-) 873 WP_274302094.1 prepilin peptidase Machinery gene
  PUP76_RS15015 (PUP76_15015) pilC 3260589..3261806 (-) 1218 WP_274302095.1 type II secretion system F family protein Machinery gene
  PUP76_RS15020 (PUP76_15020) - 3262170..3262586 (+) 417 WP_007924673.1 pilin -
  PUP76_RS15025 (PUP76_15025) - 3262872..3263228 (+) 357 WP_009045666.1 BON domain-containing protein -
  PUP76_RS15030 (PUP76_15030) - 3263431..3266349 (-) 2919 WP_274302096.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44547.46 Da        Isoelectric Point: 10.4761

>NTDB_id=790299 PUP76_RS15015 WP_274302095.1 3260589..3261806(-) (pilC) [Pseudomonas chlororaphis strain NCCB 820532]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGINPARVRKKSSSLLQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLMGELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPMFTQLVIALSEFVQHWWWLLAGALLLSMFGLR
HARRRSPRFRHWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKQAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=790299 PUP76_RS15015 WP_274302095.1 3260589..3261806(-) (pilC) [Pseudomonas chlororaphis strain NCCB 820532]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAACCCGGCCAGGGTCAGGAAGAAGTCCA
GCTCGCTGTTGCAGCGCACGCCCCCGATCAAGGCCCGCGACATCAGCCTGTTCACCAGGCAACTGGCGACCTTGATCAAG
GCCGGCGTGCCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCAGCGCGCTGCGCAAGAGGCCGCGGTACTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGGGAACAGGCCGGGGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACGCGGATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTGACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAGTTCCAGTCGATGTTCCAGGGCTTCGGCGCGCAATTGCCCATGTTCACGCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCAGGCGCGCTGTTGCTCAGTATGTTTGGCCTGCGC
CATGCCCGCCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCACTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGAGTGCCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAACCAGGTGTTCAAGCAAGCGGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCATTTTTCCATGCGCGCATCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACGGCATGCTGGAAAAGGTCGCGATCCATTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.432

100

0.654
  pilC Acinetobacter baylyi ADP1

56.127

100

0.565
  pilC Acinetobacter baumannii D1279779

55.392

100

0.558
  pilC Legionella pneumophila strain ERS1305867

52.02

97.778

0.509
  pilG Neisseria gonorrhoeae MS11

41.791

99.259

0.415
  pilG Neisseria meningitidis 44/76-A

41.687

99.506

0.415
  pilC Vibrio cholerae strain A1552

42.347

96.79

0.41
  pilC Vibrio campbellii strain DS40M4

39.055

99.259

0.388