Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   PUN35_RS16850 Genome accession   NZ_CP117986
Coordinates   3149711..3149830 (-) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus sp. N3378-2at     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 3144711..3154830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN35_RS16830 - 3145081..3147156 (-) 2076 WP_014481227.1 transglycosylase domain-containing protein -
  PUN35_RS16835 speE 3147358..3148188 (+) 831 WP_003227543.1 spermidine synthase -
  PUN35_RS16840 speB 3148249..3149121 (+) 873 WP_003227545.1 agmatinase -
  PUN35_RS16845 - 3149186..3149613 (-) 428 Protein_3275 YbaK/EbsC family protein -
  PUN35_RS16850 phrF 3149711..3149830 (-) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  PUN35_RS16855 rapF 3149814..3150959 (-) 1146 WP_014481226.1 response regulator aspartate phosphatase RapF Regulator
  PUN35_RS16860 - 3150962..3151192 (+) 231 Protein_3278 hypothetical protein -
  PUN35_RS16865 - 3151189..3152544 (+) 1356 WP_014481224.1 YncE family protein -
  PUN35_RS16870 - 3152583..3153959 (+) 1377 WP_014481223.1 YncE family protein -
  PUN35_RS16875 albG 3153965..3154666 (-) 702 WP_014481222.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=788923 PUN35_RS16850 WP_009968329.1 3149711..3149830(-) (phrF) [Bacillus sp. N3378-2at]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=788923 PUN35_RS16850 WP_009968329.1 3149711..3149830(-) (phrF) [Bacillus sp. N3378-2at]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1
  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487