Detailed information NTG0788924

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: PUN35_RS16855
• Genome accession: NZ_CP117986
• Coordinates: 3149814..3150959 (-)
• Length: 381 a.a.
• NCBI ID: WP_014481226.1
• Uniprot ID: -
• Organism: Bacillus sp. N3378-2at
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3144814..3155959

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUN35_RS16830	-	3145081..3147156 (-)	2076	WP_014481227.1	transglycosylase domain-containing protein	-
PUN35_RS16835	speE	3147358..3148188 (+)	831	WP_003227543.1	spermidine synthase	-
PUN35_RS16840	speB	3148249..3149121 (+)	873	WP_003227545.1	agmatinase	-
PUN35_RS16845	-	3149186..3149613 (-)	428	Protein_3275	YbaK/EbsC family protein	-
PUN35_RS16850	phrF	3149711..3149830 (-)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
PUN35_RS16855	rapF	3149814..3150959 (-)	1146	WP_014481226.1	response regulator aspartate phosphatase RapF	Regulator
PUN35_RS16860	-	3150962..3151192 (+)	231	Protein_3278	hypothetical protein	-
PUN35_RS16865	-	3151189..3152544 (+)	1356	WP_014481224.1	YncE family protein	-
PUN35_RS16870	-	3152583..3153959 (+)	1377	WP_014481223.1	YncE family protein	-
PUN35_RS16875	albG	3153965..3154666 (-)	702	WP_014481222.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
PUN35_RS16880	-	3154663..3155943 (-)	1281	WP_014481221.1	M16 family metallopeptidase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45627.12 Da
• Isoelectric Point: 5.1503

>NTDB_id=788924 PUN35_RS16855 WP_014481226.1 3149814..3150959(-) (rapF) [Bacillus sp. N3378-2at]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=788924 PUN35_RS16855 WP_014481226.1 3149814..3150959(-) (rapF) [Bacillus sp. N3378-2at]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	99.738	100	0.997
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572