Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PUN35_RS02075 Genome accession   NZ_CP117986
Coordinates   385491..386561 (+) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus sp. N3378-2at     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 380491..391561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN35_RS02040 - 380687..380932 (-) 246 WP_003226300.1 DUF2626 domain-containing protein -
  PUN35_RS02045 mgsR 381164..381544 (+) 381 WP_003230147.1 transcriptional regulator MgsR -
  PUN35_RS02055 rsbRD 381768..382604 (+) 837 WP_014480262.1 RsbT co-antagonist protein RsbRD -
  PUN35_RS02060 - 382657..383985 (-) 1329 WP_014480261.1 hemolysin family protein -
  PUN35_RS02065 corA 384127..385080 (+) 954 WP_014480260.1 magnesium transporter CorA -
  PUN35_RS02070 - 385082..385279 (+) 198 WP_014480259.1 CBS domain-containing protein -
  PUN35_RS02075 comGA 385491..386561 (+) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  PUN35_RS02080 comGB 386548..387585 (+) 1038 WP_031600685.1 comG operon protein ComGB Machinery gene
  PUN35_RS02085 comGC 387599..387895 (+) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  PUN35_RS02090 comGD 387885..388316 (+) 432 WP_014480256.1 comG operon protein ComGD Machinery gene
  PUN35_RS02095 comGE 388300..388647 (+) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  PUN35_RS02100 comGF 388673..389056 (+) 384 WP_014480254.1 ComG operon protein ComGF Machinery gene
  PUN35_RS02105 comGG 389057..389431 (+) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  PUN35_RS02110 - 389502..389681 (+) 180 WP_014480252.1 YqzE family protein -
  PUN35_RS02115 - 389723..390049 (-) 327 WP_003246051.1 YqzG/YhdC family protein -
  PUN35_RS02120 tapA 390321..391082 (+) 762 WP_014480251.1 amyloid fiber anchoring/assembly protein TapA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=788873 PUN35_RS02075 WP_004399124.1 385491..386561(+) (comGA) [Bacillus sp. N3378-2at]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=788873 PUN35_RS02075 WP_004399124.1 385491..386561(+) (comGA) [Bacillus sp. N3378-2at]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAAGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGTCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1