Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   PUN35_RS02080 Genome accession   NZ_CP117986
Coordinates   386548..387585 (+) Length   345 a.a.
NCBI ID   WP_031600685.1    Uniprot ID   -
Organism   Bacillus sp. N3378-2at     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 381548..392585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN35_RS02055 rsbRD 381768..382604 (+) 837 WP_014480262.1 RsbT co-antagonist protein RsbRD -
  PUN35_RS02060 - 382657..383985 (-) 1329 WP_014480261.1 hemolysin family protein -
  PUN35_RS02065 corA 384127..385080 (+) 954 WP_014480260.1 magnesium transporter CorA -
  PUN35_RS02070 - 385082..385279 (+) 198 WP_014480259.1 CBS domain-containing protein -
  PUN35_RS02075 comGA 385491..386561 (+) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  PUN35_RS02080 comGB 386548..387585 (+) 1038 WP_031600685.1 comG operon protein ComGB Machinery gene
  PUN35_RS02085 comGC 387599..387895 (+) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  PUN35_RS02090 comGD 387885..388316 (+) 432 WP_014480256.1 comG operon protein ComGD Machinery gene
  PUN35_RS02095 comGE 388300..388647 (+) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  PUN35_RS02100 comGF 388673..389056 (+) 384 WP_014480254.1 ComG operon protein ComGF Machinery gene
  PUN35_RS02105 comGG 389057..389431 (+) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  PUN35_RS02110 - 389502..389681 (+) 180 WP_014480252.1 YqzE family protein -
  PUN35_RS02115 - 389723..390049 (-) 327 WP_003246051.1 YqzG/YhdC family protein -
  PUN35_RS02120 tapA 390321..391082 (+) 762 WP_014480251.1 amyloid fiber anchoring/assembly protein TapA -
  PUN35_RS02125 sipW 391066..391638 (+) 573 WP_003230181.1 signal peptidase I SipW -
  PUN35_RS02130 tasA 391702..392487 (+) 786 WP_014480250.1 biofilm matrix protein TasA -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39592.99 Da        Isoelectric Point: 9.6259

>NTDB_id=788874 PUN35_RS02080 WP_031600685.1 386548..387585(+) (comGB) [Bacillus sp. N3378-2at]
MKKIRKVWSLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTGSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKSVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSPFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=788874 PUN35_RS02080 WP_031600685.1 386548..387585(+) (comGB) [Bacillus sp. N3378-2at]
ATGAAAAAGATTAGAAAAGTCTGGTCGTTAAAGGATCAAGCGAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACGCTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGGTTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCCATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGTGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCCTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

99.071

93.623

0.928