Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PUN35_RS01630 Genome accession   NZ_CP117986
Coordinates   305473..306090 (+) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus sp. N3378-2at     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 300473..311090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN35_RS01595 aroE 301131..301973 (+) 843 WP_014480317.1 shikimate dehydrogenase -
  PUN35_RS01600 yhbY 301967..302257 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  PUN35_RS01605 - 302269..302838 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  PUN35_RS01610 yqeK 302828..303388 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PUN35_RS01615 rsfS 303406..303762 (+) 357 WP_014480315.1 ribosome silencing factor -
  PUN35_RS01620 - 303759..304502 (+) 744 WP_014480314.1 class I SAM-dependent DNA methyltransferase -
  PUN35_RS01625 comER 304568..305389 (-) 822 WP_014480313.1 late competence protein ComER -
  PUN35_RS01630 comEA 305473..306090 (+) 618 WP_014480312.1 competence protein ComEA Machinery gene
  PUN35_RS01635 - 306157..306726 (+) 570 WP_003229978.1 ComE operon protein 2 -
  PUN35_RS01640 comEC 306730..309060 (+) 2331 WP_033881047.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PUN35_RS01645 - 309100..309234 (-) 135 WP_003229983.1 YqzM family protein -
  PUN35_RS01650 - 309275..309424 (+) 150 WP_003229985.1 hypothetical protein -
  PUN35_RS01655 holA 309464..310507 (+) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  PUN35_RS01660 rpsT 310522..310788 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=788869 PUN35_RS01630 WP_014480312.1 305473..306090(+) (comEA) [Bacillus sp. N3378-2at]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=788869 PUN35_RS01630 WP_014480312.1 305473..306090(+) (comEA) [Bacillus sp. N3378-2at]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39