Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PRJ39_RS05330 Genome accession   NZ_CP117873
Coordinates   1214401..1215516 (-) Length   371 a.a.
NCBI ID   WP_091797529.1    Uniprot ID   -
Organism   Lysobacter enzymogenes strain Lj46     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1209401..1220516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRJ39_RS05300 (PRJ39_05110) rplM 1210307..1210735 (+) 429 WP_091797519.1 50S ribosomal protein L13 -
  PRJ39_RS05305 (PRJ39_05115) rpsI 1210751..1211143 (+) 393 WP_057948911.1 30S ribosomal protein S9 -
  PRJ39_RS05315 (PRJ39_05125) - 1211557..1212066 (-) 510 WP_394539976.1 GNAT family N-acetyltransferase -
  PRJ39_RS05320 (PRJ39_05130) - 1212291..1213271 (+) 981 WP_091797524.1 fumarylacetoacetate hydrolase family protein -
  PRJ39_RS05325 (PRJ39_05135) maiA 1213536..1214198 (+) 663 WP_091797526.1 maleylacetoacetate isomerase -
  PRJ39_RS05330 (PRJ39_05140) pilU 1214401..1215516 (-) 1116 WP_091797529.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PRJ39_RS05335 (PRJ39_05145) - 1215620..1215997 (+) 378 WP_394539979.1 DUF4398 domain-containing protein -
  PRJ39_RS05340 (PRJ39_05150) - 1215994..1216620 (+) 627 WP_394539981.1 hypothetical protein -
  PRJ39_RS05345 - 1216675..1216830 (+) 156 Protein_1064 hypothetical protein -
  PRJ39_RS05350 - 1216755..1217276 (-) 522 Protein_1065 DUF6053 domain-containing protein -
  PRJ39_RS05355 (PRJ39_05155) - 1217297..1217515 (+) 219 WP_057948905.1 YdcH family protein -
  PRJ39_RS05360 - 1217773..1217928 (+) 156 WP_394539982.1 DUF6053 domain-containing protein -
  PRJ39_RS05365 (PRJ39_05160) - 1218200..1219570 (+) 1371 WP_394539983.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41025.31 Da        Isoelectric Point: 6.1908

>NTDB_id=788148 PRJ39_RS05330 WP_091797529.1 1214401..1215516(-) (pilU) [Lysobacter enzymogenes strain Lj46]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMALALPDAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKSIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSNTAGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLKAVVSQRLVVGTDGRRIPAAEVLINTPHIRDLMRRGQVHEIKQAMEESLEDGMESFDQCL
FRLYKEGKIEMEEALKAADSRDGLALKFRLSEGGTGEHDPYADMYDASAAH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=788148 PRJ39_RS05330 WP_091797529.1 1214401..1215516(-) (pilU) [Lysobacter enzymogenes strain Lj46]
ATGGACATCGGCTATTTCTTGAAGCTGATGACGGAAAAGAACGCGTCGGATATGTTCCTGACCACTGGCGCGCCGGTCTA
CATCAAGGTCGAGGGGCGCCTGTACCCGCTCGGCAACACCGGCTTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTTCCGCAGTTCGAGCGCGACCTCGAACTCAACATGGCCCTGGCCCTGCCCGACGCCGGGCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCCAGCATCGA
GGAGCTGCAGCTGCCGCAGGTGCTCAAGAGCATCATCATGGCCCCGCGCGGGCTGGTGCTGATCGTCGGCTCCACCGGCT
CGGGCAAGTCGACCACGCTGGCCTCGATGATCGACCACCGCAACAGCAACACCGCCGGCCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAAAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAAATCCTCGACGCGACCACGATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCCTCGAGCGCATC
CTCAACTTCTTCCCCGAGGCCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTCAAGGCGGTGGTGTCCCAGCG
CCTGGTGGTCGGCACCGACGGGCGCCGCATCCCCGCCGCGGAAGTGCTGATCAACACCCCGCACATCCGCGACCTGATGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAAGAGTCGCTGGAGGACGGCATGGAGTCGTTCGACCAATGCCTG
TTCCGGCTGTACAAGGAAGGCAAGATCGAGATGGAGGAGGCGCTCAAGGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCACCGGCGAGCACGATCCGTATGCGGACATGTACGACGCGTCGGCGGCGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

96.765

0.499
  pilU Acinetobacter baylyi ADP1

49.153

95.418

0.469
  pilU Vibrio cholerae strain A1552

48.588

95.418

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.723

91.375

0.418
  pilT Pseudomonas aeruginosa PAK

41.42

91.105

0.377
  pilT Acinetobacter baumannii D1279779

41.124

91.105

0.375
  pilT Acinetobacter baumannii strain A118

41.124

91.105

0.375
  pilT Acinetobacter nosocomialis M2

41.124

91.105

0.375
  pilT Acinetobacter baylyi ADP1

40.588

91.644

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.533

91.105

0.369
  pilT Legionella pneumophila strain Lp02

40.237

91.105

0.367
  pilT Legionella pneumophila strain ERS1305867

40.237

91.105

0.367
  pilT Neisseria gonorrhoeae MS11

38.617

93.531

0.361
  pilT Neisseria meningitidis 8013

38.617

93.531

0.361