Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PGW99_RS06045 Genome accession   NZ_CP117520
Coordinates   1260245..1261432 (-) Length   395 a.a.
NCBI ID   WP_273779336.1    Uniprot ID   -
Organism   Acinetobacter sp. GSS19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1255245..1266432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGW99_RS06030 - 1255751..1257187 (+) 1437 WP_273779332.1 MFS transporter -
  PGW99_RS06035 - 1257526..1258704 (+) 1179 WP_273779334.1 MFS transporter -
  PGW99_RS06040 - 1258785..1259996 (-) 1212 WP_273779335.1 YeeE/YedE family protein -
  PGW99_RS06045 pilU 1260245..1261432 (-) 1188 WP_273779336.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PGW99_RS06050 - 1261628..1262683 (-) 1056 WP_273779337.1 3-deoxy-7-phosphoheptulonate synthase -
  PGW99_RS06055 - 1263394..1264947 (+) 1554 WP_273779338.1 phospholipase D family protein -
  PGW99_RS06060 - 1265335..1265925 (-) 591 WP_273779340.1 uracil-DNA glycosylase family protein -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 44930.56 Da        Isoelectric Point: 6.3928

>NTDB_id=786225 PGW99_RS06045 WP_273779336.1 1260245..1261432(-) (pilU) [Acinetobacter sp. GSS19]
MFSPEKLEEARRYMFHMLTKVVEFGGSDLFITADFPPSIKHQGLMKQVSQQPLVPEQTKIFAYSLMNDKQRQEFERDFEC
NFAISVPDVSRFRVNVFIQQQHVGMVIRTITADIPKFESLKLPEVLKDIIMTKRGLVLMVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLATLH
ANNANQALDRIINFFPEERRNQLLMDLSANMKAIISQRLIRTKDGKGRRAAVEIMINTPLMSENILKGQFHVLKEIMAKS
RELGMQTFDQALFDLYQKDAITDEEAIRNADSANAVRLQIKLNKDNVEDDSQQAVDPFASLSIMRDEETEQIEKV

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=786225 PGW99_RS06045 WP_273779336.1 1260245..1261432(-) (pilU) [Acinetobacter sp. GSS19]
ATGTTTAGTCCTGAAAAGCTCGAAGAAGCTCGCCGATATATGTTCCATATGCTGACCAAAGTGGTTGAATTCGGTGGCTC
AGATTTGTTCATCACTGCGGACTTTCCTCCGAGTATCAAGCATCAGGGTTTAATGAAACAGGTCAGCCAGCAACCACTTG
TTCCTGAACAGACCAAAATTTTTGCCTACAGCTTAATGAACGACAAACAGCGTCAAGAGTTTGAGCGTGATTTTGAATGT
AACTTTGCGATTTCTGTTCCTGATGTTTCCCGTTTTCGTGTCAATGTGTTTATTCAGCAGCAGCATGTAGGAATGGTTAT
TCGTACCATTACGGCAGATATTCCAAAATTTGAAAGTTTGAAACTACCAGAAGTGTTAAAAGACATCATCATGACCAAGC
GTGGTCTGGTTCTGATGGTAGGTGGAACGGGATCGGGTAAATCTACATCTCTGGCGGCCATGATTGACCACCGCAATGAA
AATTCTGCAGGGCATATCATTACTGTAGAAGATCCAGTGGAATATGTACACAAGCATAAAAAGTCCATGATTACACATCG
TGAAGTGGGCGTGGATTGTCACTCTTGGCACAATGCCCTGAAAAACACCCTGCGTCAGGCCCCTGATGTAATTCTAATTG
GTGAGATTCGTGATACAGAAACCATGGAACATGCCATTGCCTTTGCGGAAACCGGTCACTTATGTCTTGCCACCTTGCAC
GCCAACAACGCCAACCAGGCTTTGGACCGTATTATCAACTTCTTCCCGGAAGAGCGCCGTAATCAGTTGTTGATGGATTT
ATCAGCCAATATGAAAGCGATTATTTCACAGCGTTTGATCCGCACCAAAGATGGTAAAGGACGTCGTGCGGCAGTGGAAA
TCATGATTAATACACCACTGATGTCGGAAAATATCCTGAAAGGCCAGTTCCACGTCCTCAAAGAAATTATGGCAAAATCA
CGTGAACTGGGTATGCAGACCTTTGACCAAGCTTTATTTGATTTGTACCAAAAAGATGCTATTACGGATGAGGAAGCGAT
CCGTAACGCAGACTCTGCCAATGCAGTCCGTTTACAGATCAAACTGAACAAGGATAACGTTGAGGATGATAGCCAACAAG
CTGTCGATCCATTTGCCAGTCTGAGCATTATGCGTGATGAAGAAACTGAGCAGATTGAAAAGGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.458

88.354

0.534
  pilU Acinetobacter baylyi ADP1

55.714

88.608

0.494
  pilU Vibrio cholerae strain A1552

50.543

93.165

0.471