Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSH68_RS02045 Genome accession   NZ_CP117464
Coordinates   472198..473523 (+) Length   441 a.a.
NCBI ID   WP_034105908.1    Uniprot ID   -
Organism   Pseudomonas lurida strain FP1465     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 467198..478523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH68_RS02025 (PSH68_02025) - 467275..469737 (-) 2463 WP_034105916.1 penicillin-binding protein 1A -
  PSH68_RS02030 (PSH68_02030) pilM 469956..470609 (+) 654 WP_305434544.1 type IV pilus biogenesis protein PilM -
  PSH68_RS02035 (PSH68_02035) - 470606..471160 (+) 555 WP_034105912.1 PilN domain-containing protein -
  PSH68_RS02040 (PSH68_02040) - 471157..472128 (+) 972 WP_305434545.1 pilus assembly protein PilP -
  PSH68_RS02045 (PSH68_02045) pilQ 472198..473523 (+) 1326 WP_034105908.1 type IV pilus secretin PilQ Machinery gene
  PSH68_RS02050 (PSH68_02050) aroK 473528..474046 (+) 519 WP_034105907.1 shikimate kinase AroK -
  PSH68_RS02055 (PSH68_02055) aroB 474172..475272 (+) 1101 WP_034105904.1 3-dehydroquinate synthase -
  PSH68_RS02060 (PSH68_02060) - 475283..476842 (+) 1560 WP_034105902.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47845.70 Da        Isoelectric Point: 4.8299

>NTDB_id=785796 PSH68_RS02045 WP_034105908.1 472198..473523(+) (pilQ) [Pseudomonas lurida strain FP1465]
MAAAVPNRVDLIQLPPPGNATSSVYSGDKLNLNFQSIELRTALQQIADVAGLNLVASDEVQGAITLRLKDVPWDQALDLV
LQAKGLDKRLKAGVLLVAPAEELAARELLTLESRKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGLEGVTDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDRSLGARWGGRLNRGHWGAGGIAKPSPEGAEPSE
NPPSSPFVDLGSLTGTSGLGIAFITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSSGATSV
SFKEASLSLEVTPQITPDDRVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=785796 PSH68_RS02045 WP_034105908.1 472198..473523(+) (pilQ) [Pseudomonas lurida strain FP1465]
ATGGCTGCTGCTGTACCCAATCGTGTAGACCTGATCCAGTTGCCACCCCCTGGCAACGCCACGTCGAGCGTCTACTCGGG
TGACAAGCTCAACCTCAACTTCCAGAGCATCGAGTTGCGCACGGCGTTGCAGCAAATTGCCGACGTGGCAGGCCTCAACC
TTGTGGCGAGTGATGAAGTGCAGGGGGCGATCACCCTGCGCCTCAAGGATGTGCCCTGGGACCAGGCGCTGGACCTGGTG
CTGCAAGCCAAAGGCCTGGATAAGCGGTTGAAAGCCGGCGTATTGCTGGTCGCACCCGCCGAAGAGTTGGCCGCGCGCGA
GCTGCTGACCCTGGAGTCGCGCAAGCAAATGGCCGAATTGGCGCCGTTGCGTCGGGAGCTGTTGCAGGTCAATTACGCCA
AGGCGGCGGACCTGGCCAAGTTGTTCCAGTCGGTCACGGGCCTGGAGGGTGTGACCGATGAGCGTGGCTCGGTGGCGGTC
GATGATCGCACCAACAACATCATCGCCTACCAGACCGGCGAGCGACTGGAAGAGCTGCGGCGGATCGTGGCGCAGCTGGA
TATTCCCGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAATGTCGATTACGACAGAAGCCTGGGTGCGCGCT
GGGGTGGGCGACTCAATCGTGGCCACTGGGGCGCTGGCGGCATTGCGAAGCCTTCGCCTGAGGGGGCTGAGCCCTCTGAG
AACCCGCCCAGCTCGCCGTTTGTCGACCTGGGGTCGCTGACCGGCACCTCCGGCCTGGGCATCGCCTTTATCACCGACAA
CCTGCTGCTGGACCTGGAACTCACCGCCATGGAAAAAACCGGCAACGGCGAAATCGTCTCGCAACCCAAGGTCGTCACTT
CCGACAAGGAAACCGCACGCATCCTCAAGGGCACCGAGATCCCCTATCAGGAATCCAGCTCCAGCGGCGCCACGTCGGTG
TCGTTCAAGGAGGCGTCGCTGTCGCTGGAGGTGACCCCGCAGATCACCCCCGACGATCGCGTGATCATGGAGGTCAAGGT
CACCAAGGATGAGCCCGACTACCTGAACAAGCTCAATGACGTGCCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGC
TGGTCAAGGATGGCGAGACCATCGTCATCGGCGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.776

99.32

0.703
  pilQ Acinetobacter baumannii D1279779

39.351

100

0.44
  pilQ Acinetobacter baumannii strain A118

39.351

100

0.44
  comQ Acinetobacter baylyi ADP1

41.556

100

0.424
  pilQ Legionella pneumophila strain ERS1305867

44.076

95.692

0.422
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.153

95.011

0.42
  pilQ Vibrio cholerae strain A1552

44.153

95.011

0.42
  pilQ Legionella pneumophila strain Lp02

43.735

95.918

0.419
  pilQ Vibrio campbellii strain DS40M4

43.735

95.918

0.419