Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PO847_RS13185 Genome accession   NZ_CP117197
Coordinates   2653136..2654206 (-) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus velezensis strain 3ZT     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2648136..2659206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PO847_RS13135 (PO847_13135) - 2648195..2648779 (-) 585 WP_022552967.1 signal peptidase I -
  PO847_RS13140 (PO847_13140) tapA 2648751..2649422 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  PO847_RS13145 (PO847_13145) - 2649681..2650010 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  PO847_RS13150 (PO847_13150) - 2650051..2650230 (-) 180 WP_022552966.1 YqzE family protein -
  PO847_RS13155 (PO847_13155) comGG 2650287..2650664 (-) 378 WP_022552965.1 competence type IV pilus minor pilin ComGG Machinery gene
  PO847_RS13160 (PO847_13160) comGF 2650665..2651060 (-) 396 WP_046559876.1 competence type IV pilus minor pilin ComGF -
  PO847_RS13165 (PO847_13165) comGE 2651074..2651388 (-) 315 WP_021494312.1 competence type IV pilus minor pilin ComGE Machinery gene
  PO847_RS13170 (PO847_13170) comGD 2651372..2651809 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  PO847_RS13175 (PO847_13175) comGC 2651799..2652107 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  PO847_RS13180 (PO847_13180) comGB 2652112..2653149 (-) 1038 WP_022552962.1 competence type IV pilus assembly protein ComGB Machinery gene
  PO847_RS13185 (PO847_13185) comGA 2653136..2654206 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  PO847_RS13190 (PO847_13190) - 2654399..2655349 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  PO847_RS13195 (PO847_13195) - 2655495..2656796 (+) 1302 WP_022552961.1 hemolysin family protein -
  PO847_RS13200 (PO847_13200) - 2656834..2657664 (-) 831 WP_022552960.1 STAS domain-containing protein -
  PO847_RS13210 (PO847_13210) - 2657871..2658251 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PO847_RS13215 (PO847_13215) - 2658488..2658733 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=782778 PO847_RS13185 WP_014418378.1 2653136..2654206(-) (comGA) [Bacillus velezensis strain 3ZT]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=782778 PO847_RS13185 WP_014418378.1 2653136..2654206(-) (comGA) [Bacillus velezensis strain 3ZT]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCAATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374