Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PQE20_RS08860 Genome accession   NZ_CP117054
Coordinates   1858761..1859354 (-) Length   197 a.a.
NCBI ID   WP_005451500.1    Uniprot ID   A0A9X3S2A2
Organism   Vibrio harveyi strain VH21FL     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1853761..1864354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQE20_RS08840 (PQE20_08840) aroB 1854630..1855730 (-) 1101 WP_038898114.1 3-dehydroquinate synthase -
  PQE20_RS08845 (PQE20_08845) aroK 1855762..1856280 (-) 519 WP_005451507.1 shikimate kinase AroK -
  PQE20_RS08850 (PQE20_08850) pilQ 1856470..1858221 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  PQE20_RS08855 (PQE20_08855) pilP 1858256..1858771 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PQE20_RS08860 (PQE20_08860) pilO 1858761..1859354 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PQE20_RS08865 (PQE20_08865) pilN 1859347..1859922 (-) 576 WP_045485665.1 PilN domain-containing protein Machinery gene
  PQE20_RS08870 (PQE20_08870) pilM 1859906..1860922 (-) 1017 WP_272759061.1 type IV pilus assembly protein PilM Machinery gene
  PQE20_RS08875 (PQE20_08875) - 1861101..1863671 (+) 2571 WP_050935720.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23020.58 Da        Isoelectric Point: 4.8068

>NTDB_id=782388 PQE20_RS08860 WP_005451500.1 1858761..1859354(-) (pilO) [Vibrio harveyi strain VH21FL]
MVNWQDLEMDEIAEWPLVPQIVVILLLVVLIQGLGYWFYLKPEQEQLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKQSEHFLYRLPLDIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVKANTYQFKPEVKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=782388 PQE20_RS08860 WP_005451500.1 1858761..1859354(-) (pilO) [Vibrio harveyi strain VH21FL]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATCGCTGAGTGGCCATTGGTGCCTCAGATAGTAGTGATCTTGTTGTT
AGTTGTGCTGATTCAAGGCCTTGGTTATTGGTTTTATTTAAAGCCAGAGCAAGAACAGCTGAATAACCTTATTCAAGAAG
AACAAACCTTAAAAGTTGCGTTACGGATCAAAGCGAACAAAGTCGCTGCTTTGCCTCAATTGCAATCGCAACTTGATGAG
CTTACTACTCGATACGAATTGCTACTGCAACAACTGCCTGCACAGAAAGAGCTGGCAACCATGTTGGCTTCGGTGAATGA
ACTCGGAATTGATAATAAGTTGACGTTCACCCGAATCGACTGGGGTGATAAGCAATCAGAACACTTCCTTTATCGTTTAC
CGCTCGACATTGAGTTGACAGGGGATTTTCATGATATCGGTCGTTTCTCCCAAGCGATTGCGACGTTGCCACGCATCATC
ACTTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTCTGCACTTTCGTGTGAAAGCAAACACTTACCA
GTTCAAACCAGAGGTGAAGAAAGATGAGAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

93.158

96.447

0.898
  pilO Vibrio cholerae strain A1552

71.066

100

0.711