Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PQE20_RS08870 Genome accession   NZ_CP117054
Coordinates   1859906..1860922 (-) Length   338 a.a.
NCBI ID   WP_272759061.1    Uniprot ID   -
Organism   Vibrio harveyi strain VH21FL     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1854906..1865922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQE20_RS08845 (PQE20_08845) aroK 1855762..1856280 (-) 519 WP_005451507.1 shikimate kinase AroK -
  PQE20_RS08850 (PQE20_08850) pilQ 1856470..1858221 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  PQE20_RS08855 (PQE20_08855) pilP 1858256..1858771 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PQE20_RS08860 (PQE20_08860) pilO 1858761..1859354 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PQE20_RS08865 (PQE20_08865) pilN 1859347..1859922 (-) 576 WP_045485665.1 PilN domain-containing protein Machinery gene
  PQE20_RS08870 (PQE20_08870) pilM 1859906..1860922 (-) 1017 WP_272759061.1 type IV pilus assembly protein PilM Machinery gene
  PQE20_RS08875 (PQE20_08875) - 1861101..1863671 (+) 2571 WP_050935720.1 penicillin-binding protein 1A -
  PQE20_RS08880 (PQE20_08880) oxyR 1863738..1864646 (-) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  PQE20_RS08885 (PQE20_08885) - 1864821..1865549 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38039.40 Da        Isoelectric Point: 6.4203

>NTDB_id=782390 PQE20_RS08870 WP_272759061.1 1859906..1860922(-) (pilM) [Vibrio harveyi strain VH21FL]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEDLSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=782390 PQE20_RS08870 WP_272759061.1 1859906..1860922(-) (pilM) [Vibrio harveyi strain VH21FL]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGATAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGATCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAGGTTGCTACCGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.533

100

0.905
  pilM Vibrio cholerae strain A1552

48.538

100

0.491