Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PPM45_RS12525 Genome accession   NZ_CP116870
Coordinates   2451392..2452009 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis subsp. subtilis strain Master_strain     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2446392..2457009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPM45_RS12495 rpsT 2446694..2446960 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  PPM45_RS12500 holA 2446975..2448018 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  PPM45_RS12505 - 2448058..2448207 (-) 150 WP_003229985.1 hypothetical protein -
  PPM45_RS12510 yqzM 2448248..2448382 (+) 135 WP_003229983.1 YqzM family protein -
  PPM45_RS12515 comEC 2448422..2450752 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PPM45_RS12520 comEB 2450756..2451325 (-) 570 WP_003229978.1 ComE operon protein 2 -
  PPM45_RS12525 comEA 2451392..2452009 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  PPM45_RS12530 comER 2452093..2452914 (+) 822 WP_004398597.1 late competence protein ComER -
  PPM45_RS12535 yqeM 2452980..2453723 (-) 744 WP_003229973.1 class I SAM-dependent DNA methyltransferase -
  PPM45_RS12540 rsfS 2453720..2454076 (-) 357 WP_003229971.1 ribosome silencing factor -
  PPM45_RS12545 yqeK 2454094..2454654 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PPM45_RS12550 nadD 2454644..2455213 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  PPM45_RS12555 yhbY 2455225..2455515 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  PPM45_RS12560 aroE 2455509..2456351 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=780727 PPM45_RS12525 WP_004398514.1 2451392..2452009(-) (comEA) [Bacillus subtilis subsp. subtilis strain Master_strain]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=780727 PPM45_RS12525 WP_004398514.1 2451392..2452009(-) (comEA) [Bacillus subtilis subsp. subtilis strain Master_strain]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39