Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PPM34_RS11870 Genome accession   NZ_CP116869
Coordinates   2323241..2323858 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis subsp. subtilis strain MGP001     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2318241..2328858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPM34_RS11840 rpsT 2318543..2318809 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  PPM34_RS11845 holA 2318824..2319867 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  PPM34_RS11850 - 2319907..2320056 (-) 150 WP_003229985.1 hypothetical protein -
  PPM34_RS11855 yqzM 2320097..2320231 (+) 135 WP_003229983.1 YqzM family protein -
  PPM34_RS11860 comEC 2320271..2322601 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PPM34_RS11865 comEB 2322605..2323174 (-) 570 WP_003229978.1 ComE operon protein 2 -
  PPM34_RS11870 comEA 2323241..2323858 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  PPM34_RS11875 comER 2323942..2324763 (+) 822 WP_004398597.1 late competence protein ComER -
  PPM34_RS11880 yqeM 2324829..2325572 (-) 744 WP_003229973.1 class I SAM-dependent DNA methyltransferase -
  PPM34_RS11885 rsfS 2325569..2325925 (-) 357 WP_003229971.1 ribosome silencing factor -
  PPM34_RS11890 yqeK 2325943..2326503 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PPM34_RS11895 nadD 2326493..2327062 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  PPM34_RS11900 yhbY 2327074..2327364 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  PPM34_RS11905 aroE 2327358..2328200 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=780642 PPM34_RS11870 WP_004398514.1 2323241..2323858(-) (comEA) [Bacillus subtilis subsp. subtilis strain MGP001]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=780642 PPM34_RS11870 WP_004398514.1 2323241..2323858(-) (comEA) [Bacillus subtilis subsp. subtilis strain MGP001]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39