Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PPM30_RS11700 Genome accession   NZ_CP116868
Coordinates   2234698..2235315 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis subsp. subtilis strain MGP003     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2229698..2240315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPM30_RS11670 rpsT 2230000..2230266 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  PPM30_RS11675 holA 2230281..2231324 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  PPM30_RS11680 - 2231364..2231513 (-) 150 WP_003229985.1 hypothetical protein -
  PPM30_RS11685 yqzM 2231554..2231688 (+) 135 WP_003229983.1 YqzM family protein -
  PPM30_RS11690 comEC 2231728..2234058 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PPM30_RS11695 comEB 2234062..2234631 (-) 570 WP_003229978.1 ComE operon protein 2 -
  PPM30_RS11700 comEA 2234698..2235315 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  PPM30_RS11705 comER 2235399..2236220 (+) 822 WP_004398597.1 late competence protein ComER -
  PPM30_RS11710 yqeM 2236286..2237029 (-) 744 WP_003229973.1 class I SAM-dependent DNA methyltransferase -
  PPM30_RS11715 rsfS 2237026..2237382 (-) 357 WP_003229971.1 ribosome silencing factor -
  PPM30_RS11720 yqeK 2237400..2237960 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PPM30_RS11725 nadD 2237950..2238519 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  PPM30_RS11730 yhbY 2238531..2238821 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  PPM30_RS11735 aroE 2238815..2239657 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=780558 PPM30_RS11700 WP_004398514.1 2234698..2235315(-) (comEA) [Bacillus subtilis subsp. subtilis strain MGP003]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=780558 PPM30_RS11700 WP_004398514.1 2234698..2235315(-) (comEA) [Bacillus subtilis subsp. subtilis strain MGP003]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39